The potential action mechanism of Huangqin-Huanglian on ulcerative colitis based on network pharmacology and molecular docking
Objective:Based on the network pharmacology method,the action mechanism of Huangqin(Radix Scutellariae)-Huanglian(Rhizoma Coptidis),the sovereign medicines of the Shaoyao decoction(芍药汤),in the prevention and treatment of ulcerative colitis by regulating intestinal flora was explored.Methods:The main active components and corresponding targets of Huangqin and Huanglian were collected from the TCMSP database,and the targets were predicted by the Swiss Target Prediction database.Ulcerative colitis and intestinal flora-related target genes were obtained from GeneCards,OMIM,and TTD databases.Venny 2.1.0 was used to obtain the intersection targets of Huangqin-Huanglian,ulcerative colitis and intestinal flora.Cytoscape 3.9.1 was used to construct a component-target network to screen core components.The STRING database and Cytoscape 3.9.1 were used to construct the target protein-protein interaction network diagram to obtain the core targets.The enrichment analysis was carried out by DAVID database,and the action mechanism was further verified by molecular docking.Results:A total of 48 active ingredients and 56 intersecting targets were obtained,the main active ingredients of Huangqin-Huanglian in the prevention and treatment of ulcerative colitis might be baicalein,quercetin,berberine,epiberberine,Muping aristolochimid,etc..The core targets were tumor protein p53,tumor necrosis factor,interleukin-6,Jun proto-oncogene,and estrogen receptor 1.The enrichment analysis showed that the action mechanism was related to pathway in cancer,tumor necrosis factor signaling pathway,interleukin-17 signaling pathway,etc.The molecular docking results showed that the core components were well bound with the core targets.Conclusion:Huangqin-Huanglian may play a role in the prevention and treatment of ulcerative colitis through multi-component,multi-target and multi-pathway by regulating intestinal flora.
万方数据
维普
