The action mechanism of the Duhuo Jizhi decoction in treating cervical spondylotic radiculopathy based on network pharmacology and molecular docking technology
Objective:To elucidate the therapeutic mechanism of the Duhuo Jisheng decoction(独活寄生汤)in treating cervical spondylotic radiculopathy(CSR)through the network pharmacology and molecular docking methodology.Methods:Active constituents and targets of the Duhuo Jisheng decoction were identified from the TCMSP database,while targets associated with CSR were screened out from GeneCards,OMIM,and DisGeNET databases.Through target mapping,potential therapeutic targets were delineated.The protein-protein interaction analysis was conducted by STRING database,and the protein-protein interaction network was constructed by Cytoscape software.Bioinformatics tools were utilized to perform GO and KEGG enrichment analyses,offering insights into the functions and pathways of prospective therapeutic targets.Molecular docking validation of active constituents was carried out by Autodock software to assess their binding affinity with target proteins.Results:The Duhuo Jisheng decoction comprised 129 active constituents,with 285 intersecting targets linked to CSR.The protein-protein interaction network analysis identified AKT1,TNF,and NFKB1 as pivotal targets in the treatment of CSR.The GO function and KEGG pathway analyses unveiled that the key targets involved PI3K-Akt signaling pathway,the regulation of apoptosis and cell proliferation and so on.Conclusion:The findings suggest that the Duhuo Jisheng decoction holds potential in ameliorating CSR symptoms by modulating inflammation and promoting cellular survival,thus furnishing theoretical underpinning for its clinical utility and paving the way for novel therapeutic approaches in managing the condition.
The Duhuo Jisheng decoctionCervical spondylotic radiculopathyNetwork pharmacologyMolecular dockingAction mechanism
万方数据
维普
