The action mechanism of Huangqi-Dilong medicine pair in the treatment of painful diabetic peripheral neuropathy based on network pharmacology and molecular docking technology
Objective:To explore the action mechanism of Huangqi(Radix Astragali)-Dilong(Pheretima)medicine pair in the treatment of painful diabetic peripheral neuropathy.Methods:The active components of Huangqi were screened through TCMSP,and the literature on the components of Dilong was searched in CNKI,Wanfang data,and VIP platforms.The screen of absorption,distribution,metabolism and excretion of exogenous chemicals in the body was performed on the obtained components,and the action targets were predicted in SwissTargetPretection website.The relevant targets of painful diabetic peripheral neuropathy were identified in GeneCards and OMIM,and Venn diagram was drew by bioinformatics platform to obtain the intersection targets.The bioinformatics GO analysis and KEGG pathway enrichment analysis were performed by David database.The drug-active component-target network map and protein-protein interaction network map were constructed using Cytoscape and STRING databases,and core targets were selected.Finally,AutoDock was used for molecular docking,and the docking results were displayed by PyMOL.Reults:A total of 47 effective active components and 631 targets of Huangqi-Dilong medicine pair,5 381 disease targets,and 391 intersection targets of medicines and diseases were obtained,and there were 77 core targets.The GO enrichment analysis mainly involved biological processes such as response to exogenous stimuli,protein phosphorylation,and protein autophosphorylation.The KEGG pathways mainly included cancer pathway,AGE-RAGE signaling pathway in diabetic complications,prostate cancer,lipid and atherosclerosis,PI3K-Akt signaling pathway,etc..Molecular docking results showed that the compounds had good binding strength with core targets,especially quercetin and TNF.Conclusion:Huangqi-Dilong medicine pair can gain the therapeutic effect on painful diabetic peripheral neuropathy through multi-component,multi-target and multi-pathway,which provides a theoretical basis and new ideas for subsequent research.
万方数据
维普
