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不同产地栀子皮和栀子仁中有效成分的含量比较

Study on Effective Constituent Contents in Different Positions of Gardenia jasminoides from Different Producing Places

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目的 对10个不同产地的栀子,不同药用部位(栀子果实、栀子皮、栀子仁)中有效成分栀子苷、西红花苷-Ⅰ、西红花苷-Ⅱ的含量进行比较,为栀子皮、栀子仁分开使用寻找科学依据.方法 采用变换波长RPHPLC法同时测定栀子苷和西红花苷-Ⅰ、西红花苷-Ⅱ的含量.Thermo ODS色谱柱C18(250 mm× 4.6 mm,5μm),甲醇-0.1%磷酸水梯度洗脱,流速1.0 mL· min-1,检测波长分别为栀子苷238 nm、西红花苷-Ⅰ和西红花苷-Ⅱ440 nm.结果 被测定成分色谱峰与其他色谱峰可以达到完全分离.栀子苷、西红花苷-Ⅰ、西红花苷-Ⅱ的进样量分别在0.420~5.252,0.199~2.495,0.023~0.288 μg范围内与各自峰面积呈良好线性关系,r分别为0.9993,0.9998,0.9997;三者平均回收率分别为98.23%,97.88%,97.77%;RSD为0.52%,2.02%,1.47%.结论 该方法简便、准确、重复性好,可同时测定多个产地栀子不同药用部位栀子有效成分的含量.为栀子皮、栀子仁分别使用提供了科学依据.
Objective To compare the contents of effective constituents of gardenoside, crocin- I and crocin- II in the fruits, seeds and pericarp of Gardeniajasminoides from different producing places. Methods Wavelength conversion RP-HPLC method was used. A Thermo ODS C18(250 mm × 4.6 mm, 5μm)column was used with the mobile phase consisting of acetonitrile-0.1%phosphoric acid( gradient elution) at the flow rate of 1.0 mL·min-1. The UV detection wavelength was set at 238 nm for gardenoside and 440 nm for crocin- I and crocin- II . Results The measured peaks were completely separated from the peaks of other components. The linear ranges of gardenoside, crocin-I and crocin-II were 0.420 ~ 5.252 μg(r=0.9993), 0.199 ~ 2.495 μg(r=0.9998) and 0.023 ~ 0.288 μg (r=0.9997), respectively. The average recovery rates of gardenoside, crocin- I and crocin-II were 98.23%(RSD=0.52%,n=6), 97.88%(RSD= 2.02% ,n -6) and 97.77% (RSD =1.47% ,n =6), respectively. Conclusion The method is sample, accurate and reproducible for the effective constituent contents in different positions of Gardenia jasminoides from different producing places.

Gardenia jasminoidesPericarp of Gardenia jasminoidesSeeds of Gardenia jasminoidesGardenosideCrocin- ICrocin- II

刘怡、陈磊、张留记

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南方医科大学中医药学院,广州510515

广东药学院中药学院,广州510006

河南省中医药研究院,郑州450004

栀子 栀子皮 栀子仁 栀子苷 西红花苷-Ⅰ 西红花苷-Ⅱ

2012

中药新药与临床药理
广州中医药大学

中药新药与临床药理

CSTPCDCSCD北大核心
影响因子:0.908
ISSN:1003-9783
年,卷(期):2012.23(1)
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