摘要
目的：探讨苍术苷（ATR）抑制氧化磷酸化电子传递链可能的作用靶点。方法将制备好的线粒体悬液分为对照组、N -乙酰- L -半胱氨酸（NAC）组、鱼藤酮（ROT）组、NAC 和 ROT 联合组，每组再分为4小组，分别用0，20，40，100μmol·L-1的 ATR 处理。测定各组线粒体呼吸链中4种蛋白质复合体和 ATP 酶的活性。结果 ATR 能够抑制复合体Ⅰ、Ⅳ和2种 ATP 酶的活性，且其抑制作用和剂量相关。结论 ATR 可能影响呼吸链电子传递的始端复合体Ⅰ、末端状态复合体Ⅳ和 ATP 酶活性，最终抑制 ATP 的形成。
Abstract
Objective To research the possible targets of atractyloside for inhibiting respiratory chain of oxidative phosphorylation. Methods The mitochondria suspension was divided into 4 groups, namely blank control group, N-acetyl-L-cysteine(NAC)group,rotenone(ROT)group,and NAC-ROT combination group. And then each group was divided into 4 subgroups,which were treated with 0,20,40,100 μmol·L-1 of atractyloside respectively. The activities of adenosine triphosphate(ATP)enzymes and 4 complexes at respiratory chain of each group were tested. Results Atractyloside could inhibit the activities of complexⅠand Ⅳ at respiratory chain,and Na+-K+-ATPase and Ca2+-Mg2+-ATPase. The effects were dose-dependent. Conclusion It suggested that atractyloside can inhibit the activities of complexⅠat initial point and ATP enzymes and complex Ⅳ at end point of respiratory chain,which results into the inhibition of ATP production.
基金项目
中国科学院重点部署项目(KSZD-EW-Z-004)
国家重点基础研究发展计划项目“973计划”项目(2009CB522804)
国家科技期刊平台
NSTL
维普
万方数据