摘要
目的 优化穿心莲种子无菌萌发的实验条件.方法 以植物组织培养技术,采用单因素法考察采收时间、 种子的成熟状态、 光照条件、 预处理、 消毒时间、 培养基对穿心莲种子萌发的影响.结果 穿心莲种子萌发的优化条件为:贮藏时间半年、 完全成熟的种子、 有光照条件、40%硫酸浸泡2 h预处理、 消毒5 min,培养基为MS或1/2 MS.结论 采用该优化条件可以在短期内培育大量整齐一致的穿心莲试管苗,且不受季节和环境条件的限制,具有较强的实用性和推广性.
Abstract
Objective To optimize the experimental conditions for aseptic germination of Andrographis paniculata (Burm. f.) Nees. seeds. Methods Plant tissue-culture technique was applied for the investigation of time after seed harvesting, seed maturity, illumination intensity, pretreatment, disinfection time and culture medium on the germination of Andrographis paniculata (Burm. f.) Nees. seeds by single factor way. Results The optimal conditions for the germination of Andrographis paniculata(Burm. f.) Nees. seeds were as follows: half a year after seed harvesting, mature seeds, light condition, soaking the seeds in 40 % H2SO4 for 2 h, disinfection for 5 min, culturing in MS or 1/2 MS medium. Conclusion Under the optimized conditions, a large number of neat test-tube seedlings were obtained after cultivating for short time without seasonal and environmental restrictions, indicating that the established method is practical and worthy of the promotion.
基金项目
广东省科技计划项目(2014A030304058)
广东省科技计划项目(2015A030302070)
广东省科技计划项目(2016A030303052)
广东省中医药强省专项资金医院中药制剂建设项目(粤中医办函[2015]102 号)
国家科技期刊平台
NSTL
维普
万方数据