首页|辛桂凝胶贴膏对糖尿病大鼠周围神经病变的影响

辛桂凝胶贴膏对糖尿病大鼠周围神经病变的影响

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目的 观察辛桂凝胶贴膏(肉桂、细辛、吴茱萸、川芎、冰片)对糖尿病大鼠周围神经病变的影响.方法 采用单次腹腔注射 1%链脲佐菌素(STZ,35 mg·kg-1)复制 2 型糖尿病大鼠模型后,结合长期(连续 8 周)高脂高糖饮食诱导产生糖尿病周围神经病变(DPN).将SD大鼠随机分为正常组、模型组、甲钴胺组及辛桂凝胶贴膏组,每组 6 只.辛桂凝胶贴膏组大鼠穴位贴敷辛桂凝胶贴膏,每天 1 次,持续给药 8 周;甲钴胺组大鼠给予甲钴胺溶液灌胃(0.045 mg·kg-1),正常组、模型组大鼠给予生理盐水灌胃.第 2、4、6、8 周测量大鼠体质量、空腹血糖(FBG);第 4、8 周检测大鼠热刺激缩足反应潜伏期(TWL);第 8 周检测大鼠的坐骨神经传导速度,包括运动神经传导速度(MNCV)及感觉神经传导速度(SNCV);采用全自动生化分析仪检测大鼠血清总胆固醇(TC)、甘油三酯(TG)、FBG水平;采用ELISA法检测空腹胰岛素(FINS)水平,计算胰岛素抵抗指数(HOMA-IR);HE染色法观察大鼠坐骨神经组织病理变化.结果 与正常组比较,模型组大鼠体质量、FINS水平显著降低(P<0.01),FBG、TC、TG水平及HOMA-IR显著升高(P<0.05,P<0.01);TWL、MNCV及SNCV显著降低(P<0.01),坐骨神经纤维排列紊乱松散,出现脱髓鞘、轴突萎缩和空泡样现象.与模型组比较,辛桂凝胶贴膏组大鼠体质量及FBG、TC、TG水平无明显变化(P>0.05),FINS水平明显升高(P<0.05),HOMA-IR明显降低(P<0.05);甲钴胺组及辛桂凝胶贴膏组大鼠的TWL、MNCV及SNCV显著升高(P<0.05,P<0.01),坐骨神经病变情况得到不同程度改善,神经纤维排列较规则,髓鞘缺失和轴突萎缩明显改善.结论 辛桂凝胶贴膏可以一定程度改善DPN大鼠的胰岛素抵抗,缓解热刺激敏感度,提高坐骨神经传导速度,对糖尿病大鼠的周围神经具有保护作用,但降糖、降血脂作用不明显.
Effect of Xin-Gui Gel Plaster on Peripheral Neuropathy in Diabetic Rats
Objective To observe the effects of Xin-Gui Gel Plaster(Cinnamomi Cortex,Asari Radix et Rhizoma,Euodiae Fructus,Chuanxiong Rhizoma,Borneolum Syntheticum)on peripheral neuropathy in diabetic rats.Methods A single intraperitoneal injection of 1%streptozotonic(STZ,35 mg·kg-1)was used to replicate a type 2 diabetes mellitus(T2DM)rat model followed by the induction of diabetic peripheral neuropathy(DPN)in combination with a long-term(8 consecutive weeks)high-fat and high-sugar diet.SD rats were randomly divided into normal group,model group,Mecobalamin group and Xin-Gui Gel Plaster group,with 6 rats in each group.The rats in the Xin-Gui Gel Plaster group were given Xin-Gui Gel Plaster at acupoints once a day for 8 weeks;the rats in the Mecobalamin group were given Mecobalamin solution by gavage(0.045 mg·kg-1),and the rats in the normal group and the model group were given physiological saline by gavage.Body mass and fasting blood glucose(FBG)were measured at weeks 2,4,6 and 8;the latency of thermal withdrawal latency(TWL)was measured at weeks 4 and 8;nerve conduction velocities,including motor nerve conduction velocity(MNCV)and sensory nerve conduction velocity(SNCV),were measured at week 8;and serum total cholesterol(TC),triglyceride(TG),fasting blood glucose(FBG)were measured using a fully automated biochemical analyzer.Fasting insulin(FINS)levels were detected by ELISA,and the index of insulin resistance(HOMA-IR)was calculated;and pathological changes in the sciatic nerve tissues of rats were observed by HE staining.Results Compared with the normal group,rats in the model group had significantly lower body mass and FINS levels(P<0.01),significantly higher levels of FBG,TC,TG and HOMA-IR(P<0.05,P<0.01);TWL,MNCV and SNCV were significantly decreased(P<0.01),and sciatic nerve fibres were disorganized and loosely aligned,with demyelination,axon atrophy and vacuole-like phenomenon.Compared with the model group,there was no significant change in body mass and levels of FBG,TC and TG in the Xin-Gui Gel Plaster group(P>0.05),FINS level was significantly increased(P<0.05),and HOMA-IR levels was significantly decreased(P<0.05);TWL,MNCV and SNCV in the Mecobalamin group and Xin-Gui Gel Plaster group were significantly increased(P<0.05,P<0.01),sciatic nerve lesions were improved to different degrees,nerve fibre arrangement was more regular,myelin deficiency and axonal atrophy were significantly improved.Conclusion Xin-Gui Gel Plaster can improve insulin resistance,relieve thermal stimulation sensitivity,improve sciatic nerve conduction velocity to a certain extent in DPN rats,and have a protective effect on peripheral nerves in diabetic rats,but the hypoglycemic and hypolipidemic effects are not obvious.

Xin-Gui Gel Plasterdiabetic peripheral neuropathythe sciatic nervenerve conduction velocityrats

谭晓、王慧雯、申奥、修彦凤、王海颖

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上海中医药大学中药学院,上海 201203

辛桂凝胶贴膏 糖尿病周围神经病变 坐骨神经 神经传导速度 大鼠

2024

中药新药与临床药理
广州中医药大学

中药新药与临床药理

CSTPCD北大核心
影响因子:0.908
ISSN:1003-9783
年,卷(期):2024.35(1)
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