首页|不同产地太白贝母中11种核苷与碱基类成分分析及产地差异研究

不同产地太白贝母中11种核苷与碱基类成分分析及产地差异研究

Compositional Analysis of 11 Nucleosides and Bases in Fritillaria taipaiensis P.Y.Li from Different Origins and the Differences in Their Origin

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目的 对 10 批采自重庆、云南、陕西等 5 个省(市)的太白贝母样品中 11 种核苷和碱基类成分的含量进行测定,采用化学计量学分析法比较太白贝母中的核苷和碱基类成分的含量差异,对其质量进行综合评价,为规范化种植和产地优选提供参考.方法 水超声提取太白贝母中的核苷和碱基类成分,采用高效液相色谱-二极管阵列检测器(HPLC-DAD)法测定样品中各成分含量,并采用主成分分析(Principal component analysis,PCA)、层次聚类分析(Hierarchical cluster analysis,HCA)对产地进行划分,偏最小二乘判别分析(Partial least squares discriminant analysis,PLS-DA)确定太白贝母中差异性的指标成分,比较指标性成分在不同产地样品间的含量差异.结果 11 种核苷和碱基类成分在不同产地太白贝母中存在显著差异;主成分分析和层次聚类分析可将样品聚为 4 类;PLS-DA鉴定出 5 个指标性成分,分别为尿嘧啶、胞嘧啶、尿苷、肌苷、腺苷,以重庆、湖北产地样品所含核苷和碱基成分相对较高,质量相对较优.结论 该方法操作简单、重复性好、准确可靠,筛选出了鉴定不同产地太白贝母中的特征性核苷和碱基类成分,可用于初步阐明不同产地样品的差异性,并能够较好地反映太白贝母的品质,为太白贝母药材采购产地选择和质量控制提供参考.
Objective The contents of 11 nucleosides and base components in 10 batches of samples from 5 provinces(cities)including Chongqing,Yunnan and Shaanxi were determined,and the differences in nucleosides and base components in Fritillaria taipaiensis were compared by chemometric analysis,and the quality was comprehensively evaluated,so as to provide a reference for the cultivation of excellent varieties and the selection of medicinal materials.Methods Nucleoside and base components were extracted from Fritillaria taipaiensis by ultrasonication in aqueous solutions,and the content of each component was determined by HPLC-DAD method.The origin was classified by principal component analysis(PCA)and hierarchical cluster analysis(HCA).Partial least squares discriminant analysis(PLS-DA)was used to determine the differentiated index components in Fritillaria taipaiensis.Then the differences in the contents of the index components among samples from different origins were compared.Results It was found that 11 nucleoside and base components differed significantly among different origins of Fritillaria taipaiensis.Principal component analysis and hierarchical cluster analysis indicated that all samples could be clustered into 4 categories.Five characteristic components,including uracil,cytosine,uridine,inosine,and adenosine,were identified by PLS-DA.The nucleosides and bases in samples from Chongqing and Hubei were relatively high,and the quality of the samples was comparatively superior.Conclusion This method is simple,reproducible,accurate and reliable.It has screened out the index nucleoside and base components in the identification of Fritillaria taipaiensis of different origins,which can be used to initially elucidate the differences of samples between different origins.Additionally,it can better reflect the quality of Fritillaria taipaiensis,and can provide reference for the selection of procurement origin and the quality control for Fritillaria taipaiensis.

Fritillaria taipaiensisgeographical originnucleosidesbasesHPLC-DADprincipal component analysishierarchical cluster analysispartial least squares discriminant analysis

梅春梅、陈富贵、赵雨薇、王丹、史长灿、邱鸿凯、周浓、李伟东

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南京中医药大学药学院中药炮制重点实验室,江苏 南京 210046

重庆三峡学院生物与食品工程学院,三峡库区道地药材绿色种植与深加工重庆市工程研究中心,重庆 404120

太白贝母 产地 核苷类 碱基类 高效液相色谱-二极管阵列检测器法 主成分分析 层次聚类分析 偏最小二乘判别分析

重庆市自然科学基金项目

CSTB2023NSCQ-LMX0010

2024

10.19378/j.issn.1003-9783.2024.03.013

中药新药与临床药理
广州中医药大学

中药新药与临床药理

CSTPCD北大核心
影响因子：0.908
ISSN：1003-9783
年,卷(期)：2024.35(3)
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