摘要
目的 建立高效液相色谱(HPLC)法同时测定黄花蒿水提浸膏中青蒿素、青蒿乙素、猫眼草黄素、猫眼草酚D的含量.方法 采用Agilent ZORBAX SB-C18(250 mm×4.6 mm,5 μm)色谱柱;流动相:乙腈(A)-纯水(B),梯度洗脱;体积流量:0.8 mL·min-1;柱温:30℃;进样量:10 μL;检测波长:210 nm.结果 青蒿素、青蒿乙素、猫眼草黄素和猫眼草酚D进样量分别在 1.608 8～16.088 μg(r=0.999 9)、0.014 1～0.141 4 μg(r=1)、0.185 1～1.850 9 μg(r=0.999 9)、0.144 1～1.441 4 μg(r=0.999 9)范围内与峰面积呈良好的线性关系,平均加样回收率(n=6)分别为 102.44%、97.82%、95.07%、95.55%,RSD分别为 1.12%、1.44%、1.29%、1.53%.结论 该方法简便准确、可靠稳定、重复性好,可用于同时测定黄花蒿水提浸膏中青蒿素、青蒿乙素、猫眼草酚D、猫眼草黄素的含量.
Abstract
Objective To establish a HPLC method for the simultaneous determination of artemisinin,arteannuin B,chrysosplenetin and chrysosplenol-D in the water extract of Artemisia annua L.Methods The analysis was performed on Agilent ZORBAX SB-C18(250 mm×4.6 mm,5 μm)column with a mobile phase of acetonitrile(A)-water(B)and the flow rate of 0.8 mL·min-1 in a gradient elution manner.The column temperature was 30℃.The injection volume was 10 μL,and the detection wavelength was 210 nm.Results Artemisinin,arteannuin B,chrysosplenetin and chrysosplenol-D were correlated well linearly with peak area in their respective ranges 1.608 8-16.088 μg(r=0.999 9),0.014 1-0.141 4 μg(r=1),0.185 1-1.850 9 μg(r=0.999 9),0.144 1-1.441 4 μg(r=0.999 9),the average recovery rate(n=6)were 102.44%,97.82%,95.07%,95.55%,and the RSD values were 1.12%,1.44%,1.29%,1.53%.Conclusion This method is convenient and accurate.It has good stability and repeatability,and can be used to simultaneously determine the content of artemisinin,arteannuin B,chrysosplenetin and chrysosplenol-D in the water extract of Artemisia annua L.
国家科技期刊平台
NSTL
万方数据