Study on the Effect and Mechanism of Eucommiae Cortex Decoction on Osteoporosis in Ovariecto-mized Rats
Objective To investigate the effect and mechanism of Eucommiae Cortex Decoction on osteoporosis in ovariectomized rats.Methods Forty-two female SD rats were randomly divided into sham operation group,model group,positive drug group(Estradiol Valerate,0.09 mg·kg-1),glutathione group(36 mg·kg-1),and low-,medium-and high-dose of Eucommiae Cortex Decoction groups(0.54,1.08,2.16 g·kg-1).After the successful replication of ovariectomized model rats,the rats were given intragastric administration at a set dose of 10 mL·kg-1,once a day for 200 days.The content of glutathione peroxidase(GSH)in serum of rats was detected by biochemical kit.The contents of tartrate-resistant acid phosphatase(TRACP)and osteoprotegerin(OPG)in serum of rats were detected by ELISA.Bone mineral density of rat femur was detected by bone densitometer.The pathological changes of tibial bone tissue in rats were observed by HE staining.The mRNA expression levels of bone morphogenetic protein 2(BMP-2),OPG and type I collagen(COL1)in bone tissue were detected by Western Blot.The mRNA expression levels of BMP-2 and Runt-related transcription factor 2(RUNX2)in bone tissue were detected by RT-PCR.Results Compared with the sham operation group,the body mass growth values of rats in the model group was significantly increased(P＜0.01).The levels of serum GSH and OPG were significantly decreased(P＜0.05,P＜0.01),and the level of TRACP was significantly increased(P＜0.01).Bone mineral density was significantly decreased(P＜0.05).The structure of trabecular bone was incomplete,with obvious fracture,and the number of trabecular bone was sparse and significantly thinned.The protein expression levels of BMP-2,OPG and COL1 in bone tissue were significantly decreased(P＜0.01).The mRNA expressions of BMP-2 and RUNX2 were decreased(P＜0.05).Compared with the model group,the body mass growth values of rats in the low-,medium-and high-dose groups of Eucommiae Cortex Decoction were significantly decreased(P＜0.05,P＜0.01),the serum GSH level was significantly increased(P＜0.01),the trabecular structure was significantly improved,the trabecular bone was thickened,the number was increased,and the mRNA expression levels of BMP-2 and RUNX2 in bone tissue were significantly increased(P＜0.01).The serum OPG level of rats in the medium-dose group of Eucommiae Cortex Decoction was significantly increased(P＜0.01).The serum TRACP level of rats in the low-dose group was significantly decreased(P＜0.01),and the protein expression level of COL1 in bone tissue was significantly increased(P＜0.05).The bone mineral density of rats in the low-and medium-dose groups of Eucommiae Cortex Decoction was significantly increased(P＜0.05,P＜0.01),and the protein expression level of OPG in bone tissue was significantly increased(P＜0.01).The protein expression level of BMP-2 in bone tissue of rats in the medium-and high-dose groups of Eucommiae Cortex Decoction was significantly increased(P＜0.05).Conclusion Eucommiae Cortex Decoction can improve the bone mineral density of ovariectomized rats,protect the integrity of trabecular bone,and has a certain preventive and therapeutic effect on osteoporosis in ovariectomized rats.The mechanism may be related to the increase of serum GSH and OPG levels,the decrease of TRACP level,the up-regulation of BMP-2,OPG,COL1 proteins and mRNA expressions of BMP-2,RUNX2,thus promoting bone formation.
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