Effect of Compound Houttuyniae Herba Extract on Sirt1/PGC-1α Pathway in Kidney Tissue of Type 2 Diabetic Rats
Objective To investigate the effect of compound Houttuyniae Herba extract(a mixture of volatile oil,water extract and alcohol extract of Arctii Fructus and Houttuyniae Herba)on Sirt1/PGC-1α pathway in kidney tissue of type 2 diabetic rats.Methods SD rats were randomly divided into normal group,model group,Rosiglitazone group(0.3 mg·kg-1),Resveratrol group(0.12 g·kg-1)and compound Houttuyniae Herba extract group(4.5 g·kg-1),with 10 rats in each group.The rat model of type 2 diabetes was replicated by high-sugar and high-fat diet for 4 weeks combined with low-dose intraperitoneal injection of 1%Streptozotocin(STZ,30 mg·kg-1).The levels of 24-hour urinary protein,24-hour urinary albumin,urinary creatinine and serum creatinine were detected,and the creatinine clearance rate and kidney index were calculated.The pathological changes of renal tissue were observed by HE staining.The expression level of Sirt1 in renal tissue was detected by immunohistochemistry.The protein expression levels of Sirt1 and PGC-1α in renal cortex of rats were detected by Western Blot.Results Compared with the normal group,the levels of 24-hour urine protein,urinary albumin,creatinine clearance rate and kidney index in the model group were significantly increased after 4 and 8 weeks of administration(P＜0.05).Glomerular volume was significantly increased,with inflammatory cell infiltration in renal interstitium and obvious expansion in mesangial area.Immunohistochemistry showed that the expression of Sirt1 in renal tissue was significantly down-regulated(P＜0.01).The protein expressions of Sirt1 and PGC-1α in renal cortex were significantly down-regulated(P＜0.05).Compared with the model group,the levels of 24-hour urinary protein and urinary albumin,creatinine clearance rate and kidney index in the compound Houttuyniae Herba extract group were significantly decreased(P＜0.05).Increased glomerular,mesangial proliferation and matrix accumulation were significantly improved.Immunohistochemistry showed that the expression of Sirt1 in renal tissue was significantly up-regulated(P＜0.01).The protein expressions of Sirt1 and PGC-1α in renal cortex were significantly up-regulated(P＜0.05,P＜0.01).Conclusion Compound Houttuyniae Herba extract has a protective effect on early renal injury in type 2 diabetic rats,and its mechanism may be related to the up-regulation of Sirt1/PGC-1α pathway in renal cortex.
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