UPLC-Q-TOF-MS/MS技术分析参苓白术散化学成分及入血成分
Analysis on Chemical Composition and Constituents Absorbed in the Blood of Shenling Baizhu San by UPLC-Q-TOF-MS/MS
李雷 1张艳丽 2陈美庆 2白珊珊 2王竹君 2方海红 1欧阳辉2
作者信息
- 1. 江西科技师范大学药学院,江西 南昌 330013
- 2. 江西中医药大学,江西 南昌 330006
- 折叠
摘要
目的 研究参苓白术散的化学成分及大鼠灌胃给药参苓白术散后大鼠血浆中的化学成分.方法 采用超高效液相色谱-四极杆/飞行时间质谱(UPLC-Q-TOF-MS/MS)技术,通过Peak View 1.2 和Chem Draw软件,根据保留时间、相对分子质量及二级离子碎片,结合对照品及文献数据,对参苓白术散的化学成分及入血成分进行鉴别.结果 从参苓白术散中鉴定出 104 个化学成分,主要为黄酮类和有机酸类成分;在灌胃后大鼠血浆中鉴定出 27 个入血成分,包括 11 个原型成分和 16 个代谢产物,主要的代谢途径为Ⅱ相代谢途径.结论 该研究通过UPLC-Q-TOF-MS/MS技术快速分析了参苓白术散的化学成分及入血成分,可为阐明参苓白术散的药效物质基础及其作用机制提供参考.
Abstract
Objective To study the chemical composition of Shenling Baizhu San and the components in rat plasma after intragastric administration of Shenling Baizhu San.Methods UPLC-Q-TOF-MS/MS was used for the analysis.According to the retention time,relative molecular weight and secondary ion fragments,as well as reference substance and literature data,the chemical composition and constituents absorbed in the blood of Shenling Baizhu San were identified by using Peak View 1.2 and Chem Draw software.Results A total of 104 chemical components were identified from Shenling Baizhu San,mainly contained flavonoids and organic acids.There were 27 components in rat plasma found after intragastric administration of Shenling Baizhu San,including of 11 prototype components and 16 metabolites.The main metabolic pathway was phase-Ⅱ metabolic pathway.Conclusion In this study,the chemical composition and constituents absorbed in the blood of Shenling Baizhu San were rapidly analyzed by UPLC-Q-TOF-MS/MS technology,which provided a reference for further elucidating the pharmacodynamic substance basis and mechanism of Shenling Baizhu San.
关键词
参苓白术散/化学成分/入血成分/代谢产物/超高效液相色谱-四极杆/飞行时间质谱Key words
Shenling Baizhu San/chemical component/constituents absorbed in the blood/metabolites/UPLC-Q-TOF-MS/MS引用本文复制引用
基金项目
国家中医药局青年岐黄学者项目(2022)(国中医药人教函[2022]256号)
江西省大学生创新创业训练计划(2023)(202310412025)
出版年
2024
NETL
NSTL
万方数据