Determination of Libanotis laticalycina Adulterated in Longze Xiongdan Capsules
Objective To establish analytical methods of Libanotis laticalycina adulterated in Longze Xiongdan Capsules.Methods Utilizing the characteristic component of Libanotis laticalycina(Xanthalin)to develop TLC method,the initial screening of the sample was performed.If the characteristic spot of Xanthalin was found,the analysis was carried out by HPLC.HPLC method was performed on SHIMADZU InertSustain® C18(250 mm×4.6 mm,5 μm)column with acetonitrile-water(58∶42)as mobile phase by isocratic elution at a flow rate of 1.0 mL·min-1.The column temperature was 30℃,the detection wavelength was set at 324 nm,and the sample injection volume was 10 μL.When the chromatographic peak area of Xanthalin in test samples was greater than that in reference samples,it suggested that Libanotis laticalycina was used to adulterate in Longze Xiongdan Capsules.If necessary,ultra-performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)could be used for further confirmation.Results TLC and HPLC methods are simple and fast.The specificity,repeatability and durability all met the requirements of"Chinese Pharmacopoeia".The result showed that Xanthalin in 19 batches of samples was detected in 107 batches of Longze Xiongdan Capsules,which indicated that there was a risk of the adulteration of Libanotis laticalycina in production of Longze Xiongdan Capsules.Conclusion The method could be applied to inspect Libanotis laticalycina adulterated in Longze Xiongdan Capsules qualitatively,which could improve the quality control of this preparation.
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