Effect of Kouqiangjie Gargle on Oral Microflora,Serum Inflammatory Factors and T Cell Subgroup Levels in Patients with Recurrent Oral Ulcer Caused by Fire Hidden in the Spleen and Stomach
Objective To explore the effects of Kouqiangjie(KQJ)Gargle on the oral microflora,serum inflammatory factors and T cell subgroup levels in patients with recurrent oral ulcer(ROU)caused by fire hidden in the spleen and stomach.Methods From April 2022 to March 2023,64 patients with ROU caused by fire hidden in the spleen and stomach,who were treated in the Department of Stomatology,the Hospital of Chengdu University of Traditional Chinese Medicine(TCM)and Department of Oral Mucosa,West China Hospital of Stomatology,Sichuan University,were enrolled as the research object.All patients were randomly divided into trial group(n=32)and control group(n=32)according to random number table method.Patients in the control group were treated with Triamcinolone Acetonide Acetate Cream,while patients in the trial group received the same treatment of the control group combined with KQJ Gargle rinsing and swallowing,3 times daily for 1 week.Clinical efficacy of the two groups were compared.The traditional Chinese medicine(TCM)syndrome scores,pain index,pain duration and ulcer healing time were recorded.The DNA concentrations of oral microflora(Streptococcus,Veillonella and Neisseria)were detected by real-time quantitative polymerase chain reaction(RT-qPCR),enzyme-linked immunosorbent assay(ELISA)was used to determine the levels of serum inflammatory factors[tumor necrosis factor-α(TNF-α),interleukin-6(IL-6)and IL-8],and flow cytometry was applied to examine the level of T cell subsets[T lymphocytes(CD3+),helper T lymphocytes(CD4+),CD4+/cytotoxic T lymphocytes(CD8+)].The adverse reactions during treatment were observed and recorded,and the recurrence rate after 6 months were also compared.Results The clinical efficacy,pain inhibitory effect and ulcer healing rate of trial group were higher than those in control group(P＜0.05).After treatment,the DNA concentrations of Streptococcus,and Veillonella were significantly higher than those before treatment in both groups.Moreover,the trial group was higher than the control group,with statistical significance(P＜0.05).The levels of serum inflammatory factors(TNF-α,IL-6 and IL-8)in two groups after treatment were diminished when compared to prior-treatment,while the levels of CD3+,CD4+ and CD4+/CD8+ were raised,and the improvement degree was greater in the trial group than in the control group.The difference was statistically significant(P＜0.05).The recurrence rate in the trial group was significantly lower than that in the control group(P＜0.05).Conclusion KQJ Gargle can significantly improve the therapeutic effect,ameliorate the compositions of oral microflora,inhibit serum inflammatory response levels,enhance the cellular immune functions,and reduce recurrence rate in the treatment of patients with ROU caused by fire hidden in the spleen and stomach,so it is worthy of clinical application.
Kouqiangjie Garglerecurrent oral ulceroral microflorafire hidden in the spleen and stomachoral microflorainflammatory factorsT cell subsets
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