Serum Metabolomic Analysis of Patients with Refractory Gout of Spleen Deficiency and Damp-Heat Type
Objective To analyze the serum differential metabolites and metabolic pathways of patients with refractory gout of spleen deficiency and damp-heat type by metabolomics based on 1 H-NMR.Methods A total of 40 patients with refractory gout acute attack of spleen deficiency and damp-heat type were selected as gout group,and 20 healthy subjects were selected as healthy group.Erythrocyte sedimentation rate and serum C-reactive protein(CRP),interleukin-6(IL-6),tumor necrosis factor-α(TNF-α),serum adiponectin and creatinine levels were used as observation indicators.Serum metabolomics analysis was performed using 1 H-NMR method.The obtained data were subjected to principal component analysis(PCA)and orthogonal partial least squares discriminant analysis(OPLS-DA)to determine the corresponding metabolites and analyze the potential metabolic pathways of the differential metabolites.Results Compared with the healthy group,the erythrocyte sedimentation rate,serum CRP,IL-6,TNF-α and other inflammatory indicators and cell chemokine levels in the gout group were significantly increased(P＜0.01);the serum adiponectin level was significantly decreased(P＜0.01),and the serum creatinine level was significantly increased(P＜0.01).There were significant differences in the content of serum metabolites between the healthy group and the gout group.A total of 44 differential metabolites were obtained,involving 12 potential metabolic pathways such as aminoacyl tRNA biosynthesis,valine,leucine and isoleucine biosynthesis,alanine,aspartic acid and glutamic acid metabolism,glycolysis/gluconeogenesis,ketone synthesis and degradation,citric acid cycle(TCA cycle),glutathione metabolism,glyceride metabolism.Conclusion Acute attacks of refractory gout with spleen deficiency and damp-heat type may be associated with disorders of mitochondrial energy metabolism,leading to abnormalities in glucose metabolism,lipid metabolism,and amino acid metabolism.
NETL
NSTL
万方数据
