To Explore the Effect of Yifei Jianpi Prescription on Airway Mucus Hypersecretion in COPD Model Rats Based on EGFR/MAPK/MUC5AC Pathway
Objective To explore the therapeutic effects and mechanism of Yifei Jianpi Prescription on airway mucus hypersecretion in chronic obstructive pulmonary disease(COPD)rats through the epidermal growth factor receptor/mitogen-activated protein kinase/mucin 5AC(EGFR/MAPK/MUC5AC)pathway.Methods Male SD rats were randomly divided into blank group,model group,ambroxol group and Yifei Jianpi Prescription low-,medium-and high-dose groups.The COPD rat model with lung and spleen deficiency was replicated by smoking combined with lipopolysaccharide instillation and administration of Folium Sennae.Alcian blue-periodic acid-Schiff staining(AB-PAS)was used to observe bronchial mucus secretion and the degree of goblet cell hyperplasia in rats.Hematoxylin-eosin staining(HE)was used to observe the pathological morphology of lung tissue of rats.Western Blot was used to detect the expression of MUC5AC,EGFR protein,c-Jun N-terminal kinase(JNK),p38 MAPK,protein and phosphorylated protein of extracellular regulated protein kinase(ERK)in rat lung tissues.Real-time fluorescence quantitative PCR(RT-qPCR)was used to detect the mRNA expression of EGFR,JNK,p38 MAPK,MUC5AC,and ERK in rat lung tissues.Results AB-PAS staining showed that the number of goblet cells decreased significantly in ambroxol group and all dose groups of Yifei Jianpi Prescription.HE staining showed that ambroxol group and Yifei Jianpi high-dose group significantly improved the pathological injury of lung tissue.Compared with the blank group,the mRNA expressions of ERK,MUC5AC,p38 MAPK,JNK and EGFR were significantly increased in the lung tissues of the model group(P＜0.01),and the expression of MUC5AC and EGFR protein,as well as p38 MAPK,JNK,ERK total and phosphorylated protein were significantly increased(P＜0.01).Compared with model group,the protein expression levels of ambroxol group and Yifei Jianpi Prescription medium-and high-dose groups were significantly decreased(P＜0.01,P＜0.05),while the protein expression levels of EGFR,p38 MAPK and p-p38 MAPK in Yifei Jianpi Prescription low-dose group were decreased(P＜0.01,P＜0.05).The mRNA expression of EGFR,ERK,JNK,p38 MAPK and MUC5AC in Yifei Jianpi Prescription high-dose and ambroxol groups were decreased(P＜0.01,P＜0.05),and the mRNA expression of MUC5AC in medium-and low-dose groups were decreased(P＜0.05).Conclusion Yifei Jianpi Prescription can reduce the expression of MUC5AC and improve hypersecretion of airway mucus.Its mechanism is related to the EGFR/MAPK signaling pathway.
Chronic obstructive pulmonary diseaselung and spleen deficiencyMUC5ACEGFRMAPKYifei Jianpi Prescriptionairway mucusrats
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