Study on the Protective Effect and Mechanism of Xiaoer Kaiwei Zengshi Mixture on Intestinal Mucosal Barrier in Young Rats with Anorexia
Objective To study the protective effect and mechanism of Xiaoer Kaiwei Zengshi Mixture (Atractylodis Rhizoma,Foria,Calli Gigerii Endothelium Corneum,Mume Fructus,etc.)on the intestinal mucosal barrier of young rats with anorexia. Methods A total of 50 SD young rats were randomly divided into normal group,model group,probiotic group(Bifidobacterium quadruplex viable tablets,0.405 g·kg-1),Xiaoer Kaiwei Zengshi Mixture high-and low-dose groups (11.4,5.7 g·kg-1),with 10 rats in each group. The etiological simulation method was used to establish a young rat model of anorexia,and the modeling effect was evaluated by recording changes in food intake and body mass. After intragastric administration(10 mL·kg-1,once a day)for 42 days,the pathological changes of colon tissue were observed by HE staining. The ultrastructure of intestinal mucosal barrier was observed by transmission electron microscopy. The level of serum IL-22 was detected by ELISA. The mRNA and protein expressions of Claudin-1,Occludin,and ZO-1 in the colon tissues were detected by RT-PCR and Western Blot. Results Compared with the normal group,rats in the model group exhibited a significant decrease in food intake and body mass (P<0.05)and severe damage to the colonic mucosa,with partial rupture of intercellular junctions in the colonic mucosa and decreased density of desmosomes. Serum IL-22 level was significantly reduced(P<0.01). The mRNA and protein expressions of Claudin-1,Occludin,and ZO-1 in the colonic tissues were significantly down-regulated(P<0.01). Compared with the model group,the food intake and body mass of the rats in the treatment groups were significantly increased (P<0.05). It has been shown that the degree of damage to the colonic mucosal was reduced,and the intercellular junctions of the colonic mucosa were repaired in various degrees. The level of serum IL-22 was significantly increased(P<0.01),and the protein expressions of Occludin,and ZO-1 in the colonic tissues were significantly up-regulated (P<0.05,P<0.01). The mRNA expressions of Claudin-1,Occludin,and ZO-1 and the protein expression of Claudin-1 of the colonic tissues in probiotic group and Xiaoer Kaiwei Zengshi Mixture high-dose group were significantly up-regulated (P<0.05,P<0.01). Conclusion Xiaoer Kaiwei Zengshi Mixture has a protective effect on the intestinal mucosal barrier of young rats with anorexia by regulating IL-22 level and the expression of intestinal barrier-related tight junction proteins,including Claudin-1,Occludin,and ZO-1,thereby increasing food intake and body mass.
anorexia in childrenXiaoer Kaiwei Zengshi Mixtureintestinal mucosal barriertight junction proteinIL-22rats
万方数据
维普
