Transcriptome Analysis in Mesona Chinensis Benth at Different Flowering Developmental Stages and Mining of Genes Related to Flavonoid Synthesis
Objective To explore the key genes in the flavonoid metabolic pathway of Mesona chinensis Benth and to compare the changes of related genes in leaves at different flower developmental periods (budding,blossoming and withering). Methods Leaves of two cultivars of M. chinensis,Pingyuan grass and Taiwan grass,were used as test materials at three periods (budding,blossoming,and withering),and were subjected to transcriptome sequencing and bioinformatics analyses using the Illumina NovaSeq 6000 sequencing platform. Results A total of 99945 unigenes were obtained from transcript data assembly and de-redundancy,of which 56362 unigenes were successfully annotated. 30116 unigenes were compared with the KOG database to obtain annotations,and the general function prediction was the most numerous group. 44461 unigenes were compared in the GO database to obtain annotations. These unigenes were classified into three GO categories:biological processes,cellular components,and molecular functions according to the functions,with 57 subgroups. The screening yielded 7466 differentially expressed genes,and KEGG enrichment analysis showed that the differential genes were mainly enriched in the pathways of plant-pathogen interactions,plant hormone signal transduction,MAPK signaling pathway-plant,and phenylpropanoid biosynthesis. A total of 51 differentially expressed genes were related to flavonoid biosynthesis. It was found that the gene expressions of the two cultivars were basically the same in the same period with the help of gene expression analysis,and the expression differences were significant at budding,blossoming and withering stages. Five differential genes were randomly selected for RT-qPCR verification,and the results showed that the gene expression results were basically consistent with the transcriptome expression profile. Conclusion The study identified the key genes of flavonoid metabolism pathway and their expression patterns in the leaves of the two cultivars during the various stages of flower development,which provided a theoretical basis for the exploration of flavonoid biosynthesis in M. chinensis.
万方数据
维普
