Effect of Di'ao Xinxuekang on the Gut Microbiota in Atherosclerosis Model Mice
Objective To observe the effect of Di'ao Xinxuekang (XXK)on gut microbiota in atherosclerosis (AS) model mice. Methods ApoE-/-mice were fed a high-fat diet to establish the AS model,concurrently administered with XXK (80,160 mg·kg-1) and simvastatin (1.3 mg·kg-1). After 18 weeks of the treatment,serum levels of total cholesterol(TC),triglycerides(TG),low-density lipoprotein cholesterol(LDL-C),and high-density lipoprotein cholesterol(HDL-C)were measured in mice. Oil Red O staining was used to observe the formation of aortic plaque and hepatic lipid droplets. 16S rRNA microbiota diversity analysis was performed to study changes of fecal microbiota in mice. Results Comparison with the model group,serum levels of TC,TG,and LDL-C in XXK high-dose group and simvastatin group were significantly reduced (P<0.05,P<0.01),while HDL-C levels were significantly increased (P<0.05,P<0.01). The formation of aortic plaque and hepatic lipid droplets was significantly inhibited to varying degrees (P<0.05,P<0.01). Alpha and beta diversity analyses indicated good separation in sample clusters of each group. Compared with the control group,the abundance of Akkermansia in the model group significantly decreased(P<0.01). But the treatment with XXK increased the abundance of Akkermansia (P<0.05),which negatively correlated with the levels of TC,TG and LDL-C,and hepatic lipid droplet area (P<0.05,P<0.01). Conclusion The lipid lowering and anti-AS effects of XXK may be related to its modulation of gut microbiota dysbiosis and enhancement of the abundance of the beneficial bacterium Akkermansia.
万方数据
维普
