Explore the Mechanism of Qingchang Xiaopi Decoction in the Treatment of Dextran Sulfate Sodium-induced Ulcerative Colitis Mice Model Based on Intestinal Flora and Bile Acid Metabolism
Objective To study the therapeutic effect of Qingchang Xiaopi Decoction on ulcerative colitis (UC) induced by dextran sulfate sodium (DSS). Methods C57BL/6 mice were randomly divided into four groups:normal group,model group,Qingchang Xiaopi Decoction group (14.71 g·kg-1·d-1) and mesalazine group (1 g·kg-1·d-1),with 10 mice in each group. The mice in the model group and the drug administration group freely drank 3％ DSS for seven consecutive days,and then the UC mouse model was established. The normal group was given normal drinking water. At the same time,the model mice in each group were given the corresponding drug lavage according to the body mass of 10 mL·kg-1 dose for 10 consecutive days. The general condition,body mass and DAI score of mice were observed and recorded every day. HE staining method was used to observe the pathological changes of colon tissue,and pathological score was carried out. 16S rDNA sequencing technology was used to detect the structural changes of intestinal flora in mice,and targeted bile acid metabolomics was used to detect the changes of bile acid content in mice feces. Results Qingchang Xiaopi Decoction could alleviate the decrease of body mass (P<0.01),increase of DAI score,decrease of spleen index,increase of thymus index,shortening of colon length and decrease of colon pathological score (P<0.05,P<0.01)in DSS-induced UC mice. Qingchang Xiaopi Decoction could increase the relative abundance of Firmicutes,reduce the relative abundance of Bacteroidetes and Proteobacteria,and increase the relative abundance of Muribaculaceae,Lachnospiraceae NK4A136,Ruminococcus (P<0.05,P<0.01). In addition,Qingchang Xiaopi Decoction could decrease the content of β-MCA,CA,CDCA,Tβ-MCA,and increase the content of ω-MCA,deoxycholic acid (DCA),and TDCA in feces of mice (P<0.05,P<0.01). Finally,the correlation analysis of colitis,intestinal flora and bile acid metabolism found that there was a significant positive correlation between Muribaculaceae,Lachnospiraceae NK4A136,Ruminococcus and DCA,lithocholic acid(LCA)in secondary bile acids. Conclusion Qingchang Xiaopi Decoction can regulate the disturbance of bile acid metabolism mediated by intestinal flora and reduce intestinal inflammation by regulating the structural changes of intestinal flora,so as to play a role in the treatment of DSS-induced UC mice.
万方数据
维普
