Simultaneous Determination of Six Active Components in Chaige Hangover-alleviating Granules by HPLC
Objective HPLC method was established to simultaneously determine the content of six active components including puerarin,dihydromyricetin,tectoridin,kakkalide,quercetin,and saikosaponin A in Chaige Hangover-alleviating Granules (CAG),and to provide a reference for the improvement of its quality standard. Methods The separation was performed on an Agilent 5HC-C18 column (4.6 mm × 250 mm,5 μm)with 0.2％ phosphoric acid in water (A)-acetonitrile (B) as the mobile phase in a gradient elution at a flow rate of 1.0 mL·min-1. The column temperature was 30 ℃,the detection wavelength was 210 nm,and the injection volume was 10 μL. Results A good separation of puerarin,dihydromyricetin,tectoridin,kakkalide,quercetin,and saikosaponin A in CAG could be achieved under the above chromatographic conditions,with the linear ranges of 21.16～677.00 μg·mL-1(r=1.0000),11.16～357.00 μg·mL-1(r=0.9997),19.28～617.00 μg·mL-1(r=0.9999),10.38～332.00 μg·mL-1(r=0.9999),15.38～492.00 μg·mL-1 (r=0.9998),and 15.50～496.00 μg·mL-1 (r=0.9996)respectively,showing a good linear correlation. The average recoveries ranged from 98.43％～101.35％,and the RSDs were all less than 3％. Conclusion The method can simultaneously determine the content of puerarin,dihydromyricetin,tectoridin,kakkalide,quercetin,and saikosaponin A in the CAG. Moreover,the established method is accurate,reproducible and operable,which provides the basis for the quality evaluation and control of CAG.
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