血铅的石墨炉原子吸收直接测定法
Direct determination of blood lead by graphite furnace atomic absorption spectrometry
张道玉 1邱倩1
作者信息
- 1. 连云港市赣榆区疾病预防控制中心检验科,江苏连云港 222100
- 折叠
摘要
目的 建立适合检测铅作业工人血铅浓度的石墨炉原子吸收法.方法 根据中华人民共和国行业标准,血中铅的石墨炉原子吸收光谱测定法进行血铅检测,同时研究了仪器条件、取样量、基体改进剂磷酸二氢铵等分析条件对测定结果的影响.结果 0.1 mL全血经0.9 mL 5％硝酸酸化后,使用50 g/L磷酸二氢铵基体改进剂进行测定,溶液浓度在0～50 µg/L范围内,标准曲线线性良好,r=0.999 6;样品相对标准偏差(RSD)在1.70％～5.75％,方法的检出限为2.0 μg/L,与行标的操作方法比较,标准品的检测结果令人满意.结论 所建立的方法具有良好的灵敏度、准确度,可适用于大批量铅作业工人血铅的测定.
Abstract
Objective To establish a graphite furnace atomic absorption method suitable for detecting blood lead concentration in lead workers.Methods According to the industrial standard of the People's Republic of China,the blood lead was determined by graphite furnace atomic absorption spectrometry,and the influence of instrument conditions,sampling amount and matrix modifier dihydrogen phosphate on the determination results was studied.Results After 0.1 mL whole blood was acidified by 0.9 mL 5％nitric acid,the solution concentration was determined by 50 g/L dihydrogen phosphate as matrix modifier.The standard curve was linear in the range of 0-50 μg/L,r=0.999 6.The relative standard deviation of the samples ranged from 1.70％to 5.75％,and the detection limit of the method was 2.0 μg/L,which was satisfactory compared with the standard method.Conclusion The method has good sensitivity and accuracy,and can be used for the determination of blood lead in large quantities of lead workers.
关键词
石墨炉原子吸收法/血铅/磷酸二氢铵/铅作业工人Key words
Graphite furnace atomic absorption method/Blood lead/Dihydrogen phosphate/Lead worker引用本文复制引用
出版年
2024
NETL
NSTL
万方数据