Effects of 1-nitropyrene on lifespan of Caenorhabditis elegans through autophagy pathway
Objective By using the Caenorhabditis elegans model(hereafter referred to as C.elegans),to investigate the effects of 1-nitropyrene(1-NP)on the lifespan of C.elegans and the role of autophagy in the impact of lifespan.Methods The effects of subacute exposure(five days)of 1-NP(0,10-4,10-2,102,104 ng/L)on the life span of C.elegans were studied under normal culture conditions.The effects of 1-NP on health indicators(body length,exercise capacity and lipofuscin)and longevity of C.elegans were observed.The expression of autophagy related marker genes(bec-1 and lgg-1)was detected by quantitative real time-polymerase chain reaction(qRT-PCR).Results Compared with the control group,1-NP could significantly reduce the lifespan of C.elegans,and 104 ng/L group had the most significant effect,with a reduction rate of 28.2％.1-NP(104 ng/L)decreased the body length of C.elegans(P＜0.05)and the expression levels of key autophagy genes(bec-1 and lgg-1)(P＜0.05),increased lipofuscin accumulation in C.elegans(P＜0.05).Conclusion 1-NP can shorten the lifespan of C.elegans by decreasing the autophagy level in C.elegans.
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