Determination of 14 mycotoxins in hams with QuEchER based extraction and liquid chromatography tandem mass spectrometry
Objective To establish a QuEchERs extraction and ultra high performance liquid chromatography tandem triple quadrupole mass spectrometry(LC-MS/MS)method for the determination of 14 mycotoxins(fumarins B1,fumarins B2,fumarins B3,aflatoxin B1,aflatoxin B2,cyclopianic acid,aflatoxin G1,aflatoxin G2,citronillin,ophiformin,T2 toxin,Neosolanol,Sterigmatocystin,and Ochratoxin A)in hams.Methods The ham samples were extracted by 20 mL 80％acetonitrile(acetonitrile+water+formic acid=80+19+1).The extracts were purified by QuEchERs,and the mobile phases were 0.1％formic acid water and acetonitrile.The samples were separated by C18 column and quantitated by multiple reaction monitoring(MRM).Results After testing,the toxin content of 14 mycotoxins ranged from 0.5 to 100 μg/L,with correlation coefficients＞0.997.The lower limit of quantification(LOQ)were 1.67 to 66.67 μg/kg.In addition,the spiked recoveries was 79.1％-96.2％,and the relative standard deviation were 1.1％-9.5％.Conclusion The QuEchERs extraction ultra-high performance liquid chromatography tandem quadrupole mass spectrometry detection method established is fast,accurate,sensitive,and suitable for the detection of 14 mycotoxins in ham.
HamMycotoxinUltra high performance liquid chromatography tandem quadrupole mass spectrometry
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