Simultaneous determination of trans-trans mucofuric acid and phenylthiouric acid in urine by online solid-phase extraction liquid chromatography mass spectrometry
Objective To establish an online solid-phase extraction liquid chromatography-mass spectrometry method for the simultaneous determination of trans-trans mucofuric acid and phenylmercaptouric acid in urine.Methods The urine samples were centrifuged and processed on the machine,purified and enriched by online solid-phase extraction on HLB column(20 mm×3.9 mm×15 μm),and analysed by C18 column(150 mm×4.6 mm×5 μm),detec ted by linear ion trap mass spectrometry.Results The linear range of trans-trans mucofuric acid and phenylmercaptouric acid were 0.0-15.0 mg/L and 0.0-500.0 μg/L,with correlation coefficients of 0.999 8 and 0.999 9,and detection limits of 3.5 and 0.1 μg/L,the recovery rates were 103.3％ -108.8％ ,and the relative standard deviations were 2.8％ -8.4％ .Conclusion The sample pre-treatment of this method only requires centrifuged,which is simple and fast,with accurate results,good reproducibility and high sensitivity,and it was suitable for the detection of biological monitoring indicators in large quantities of occupational benzene exposure population.
NETL
NSTL
万方数据
