Determination of malachite green in aquatic products by boron doped molecularly imprinted polysiloxanemer modified electrochemical sensor
Objective To establish a new method for the determination of malachite green(MG)in aquatic products using the electrochemical sensor that was constructed by modifying carbon paste electrode(CPE)using boron doped molecularly imprinted polysiloxanemer(BMIP).Methods BMIP was synthesized using phenyltrimethoxysilane and methyltrimethoxysilane as functional monomers,MG as template molecule,meta aminophenylboronic acid as dopant,and tetraethoxysilane as crosslinking agent.BMIP was used to modify CPE and construct electrochemical sensor(BMIP-CPE)for detecting MG content in aquatic products.Results By optimizing the dosage of functional monomers,dopants,crosslinking agents,eluents,enrichment time and other experimental parameters,under optimal conditions,the linear range of MG in aquatic products was determined to be 0.2-60.0 μmol/L using differential pulse voltammetry,with a correlation coefficient of 0.999 2 and a detection limit of 0.075 μmol/L.In addition,the BMIP-CPE showed enough stability and good reproducibility.This method was applied to the detection of MG in commercially available aquatic products such as fish and shrimp,with an acceptable recoveries of 90.2％to 97.8％.The results were compared with high performance liquid chromatography,and the difference was not statistically significant(P＞0.05).Conclusion The BMIP modified electrochemical sensor is a simple and fast method for detecting MG in aquatic products,with good linear range,high specificity and accuracy,and is suitable for the determination of MG in aquatic products.
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