Effect of occupational nickel exposure on malignant biological behavior of colorectal cancer SW620 cells:Based on the miR-101-5p-ESR1 pathway
Objective Based on microRNA-101-5p(miR-101-5p)-estrogen receptor(ESR1)pathway,to explore the effect of occupational nickel exposure on malignant biological behavior of colorectal cancer SW620 cells.Methods The colorectal cancer SW620 cell group,low-dose,medium-dose,and high-dose groups exposed to nickel(NiSO4final concentrations were 200.0,400.0,and 800.0 mg/mL)were set up in an environment of 37 ℃,5％CO2,and 95％humidity.After culturing for 72 hours,the cell viability was measured by methyl thiazolyl tetrazolium(MTT)method,the number of single clones was measured by crystal violet staining,the cell apop-tosis was detected by flow cytometry,the level of cell invasion and migration was measured by matrigel coating membrane and scratch test,and the levels of miR-101-5p and ESR1 were determined by real time fluorescence quantitative PCR and Western blot.Results Compared with the colorectal cancer SW620 cell group,the survival rate,colony formation number,membrane pene-tration number,migration distance,ESR1 mRNA protein expression level of low,medium and high dose NiSO4 exposure groups[(1.39±0.22)vs(1.91±0.31),(3.27±0.50),(5.89±0.47)and(0.45±0.07)vs(0.88±0.11),(1.25±0.13),(1.45±0.18)]increased,while the apoptosis rate,miR-101-5p expression level[(3.58±0.36)vs(2.85±0.21),(2.29±0.19),(1.15±0.08)]decreased,and the differences were statistically significant(all P＜0.05).With the gradual increase of NiSO4 exposure dose level,the survival rate,colony formation number,membrane penetration number,migration distance,ESR1 mRNA protein expression level of each dose group exposed to NiSO4[(1.91±0.31)vs(3.27±0.50)vs(5.89±0.47),(0.88±0.11)vs(1.25±0.13)vs(1.45±0.18)]gradually increased,the apoptosis rate,miR-101-5p expression level[(2.85±0.21)vs(2.29±0.19)vs(1.15±0.08)]gradually decreased,and the differences were statistically significant(all P＜0.05).Conclusion Nickel inhibits the expression of miR-101-5p in colorectal cancer cells and promotes the high expression of ESR1,thereby promoting the proliferation,invasion and migration of colorectal cancer cells,and inhibiting apoptosis.
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