Simultaneous determination of 11 β2-receptor agonists and 4β-receptor blockers in meat product based ulra-high performanee liquid chromatography tandem triplequadrupole mass speclrometry
Objective To establish a novel,rapid,and accurate ulra-high performanee liquid chromatography tandem triplequadrupole mass speclrometry(LC-MS/MS)detection method for the simultaneous detection of 11 β2-receptor agonists(tobuterol,marbuterol,fenoterol,zilpaterol,pentbuterol,brobuterol,bambuterol,salbutamol,ractopamine,terbutaline,clenbuterol)and 4 β-recepltor blockers(propranolol,natolol,carazololum,atenolol)in meat products.Methods The meal products were extracted by 5％trichloroacetic acid(TCA),purilied on a Waters Oasis MCX column,and the mobile phases were 0.1％formic acid water+10 mmol/L ammonium acetate.The sample were separated by C18 column by gradient elution method,and quantitated by muliplereaction detection mode.Results The linear relationship of 11 β2-receptor agonists and 4 β-receptor blockers showed good stability at 0.5-20 mg/L,with a correlation coefficient r＞0.999 0.The spiked recovery rate of the method was 58.5％-1 13.2％,and the relative standard deviation was 0.3％-4.9％.Conclusion The established method is highly accurate,sensitive and suitable for the detection of 11 β2-receptor agonists and 4 β-receptor blockers in meat products.
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