超高效液相色谱串联四级杆质谱法同时测定肉制品中11种β2-受体激动剂及4种β-受体阻断剂多组分残留
Simultaneous determination of 11 β2-receptor agonists and 4β-receptor blockers in meat product based ulra-high performanee liquid chromatography tandem triplequadrupole mass speclrometry
白应皓 1栾杰2
作者信息
- 1. 红河州疾病预防控制中心理化检验所,云南蒙自 661100
- 2. 云南省疾病预防控制中心理化检验所,云南 昆明 650022
- 折叠
摘要
目的 建立一种新型、快速、准确的超高效液相色谱串联四级杆质谱检测方法,用于同时检测肉类食品中11种β2-受体激动剂(妥布特罗、马布特罗、非诺特罗、奇帕特罗、喷布特罗、溴布特罗、班布特罗、莱克多巴胺、沙丁胺醇、特布他林、克仑特罗)和4种β-受体阻断剂(普萘洛尔、纳多洛尔、卡拉洛尔、阿替洛尔).方法 肉制品经5％三氯乙酸(trichloroacetic acid,TCA)提取,Waters Oasis MCX柱净化,0.1％甲酸水+10 mmol/L乙酸铵和甲醇为流动相,梯度洗脱方式,经C18柱分离,采用多重反应检测模式进行定量检测.结果 11种β2-受体激动剂和4种β-受体阻断剂的线性关系在0.5～20 μg/L范围内表现出良好的稳定性,其相关系数r＞0.999 0,而且方法的加标回收率为58.5％～113.2％,相对标准偏差为0.3％～4.9％.结论 所建立的方法准确、灵敏,适合于肉制品中11种β2-受体激动剂和4种β-受体阻断剂的检测.
Abstract
Objective To establish a novel,rapid,and accurate ulra-high performanee liquid chromatography tandem triplequadrupole mass speclrometry(LC-MS/MS)detection method for the simultaneous detection of 11 β2-receptor agonists(tobuterol,marbuterol,fenoterol,zilpaterol,pentbuterol,brobuterol,bambuterol,salbutamol,ractopamine,terbutaline,clenbuterol)and 4 β-recepltor blockers(propranolol,natolol,carazololum,atenolol)in meat products.Methods The meal products were extracted by 5％trichloroacetic acid(TCA),purilied on a Waters Oasis MCX column,and the mobile phases were 0.1％formic acid water+10 mmol/L ammonium acetate.The sample were separated by C18 column by gradient elution method,and quantitated by muliplereaction detection mode.Results The linear relationship of 11 β2-receptor agonists and 4 β-receptor blockers showed good stability at 0.5-20 mg/L,with a correlation coefficient r＞0.999 0.The spiked recovery rate of the method was 58.5％-1 13.2％,and the relative standard deviation was 0.3％-4.9％.Conclusion The established method is highly accurate,sensitive and suitable for the detection of 11 β2-receptor agonists and 4 β-receptor blockers in meat products.
关键词
β2-受体激动剂/β-阻断剂/超高效液相色谱串联三重四级杆质谱法/多组分残留Key words
β2-receptor agonists/β-receptor blockers/Ulura-high performance liquid chromatography tandem triple quadrupolemass spectrmetry/Multi component residue引用本文复制引用
出版年
2024
NETL
NSTL
万方数据