Characteristics of drug resistance gene mutations in 156 clinical isolates of mycobacterium tuberculosis from Tianjin Center for Tuberculosis Control
Objective To analyze the characteristics of resistance mutations in genes rpoB for rifampin(RFP),katG,inh A and aphC for isoniazid(INH)in clinical isolates of mycobacterium tuberculosis complex(MTBC),and provide theoretical basis for formulating precise treatment plans for drug-resistant tuberculosis patients.Methods From October 2021 to January 2024,712 MTBC isolates from tuberculosis patients in Tianjin Center for Tuberculosis Control were collected,and the drug-resistant mutations were detected by fluorescence PCR melting curve method.Results Among 712 strains of MTBC,a total of 156 strains with drug-resistant gene mutations were detected.Among them,88 strains had mutations in the rpoB gene,86 strains had mutations in the katG315 gene site,40 strains had mutations in the inhA gene,and 9 strains had mutations in the aphC gene.Among the strains with mutations resistant to PFP molecules,a total of 9 mutation types were detected,mainly characterized by single point mutations,accounting for 93.18％.Among them,rpoB529-533 was the most important mutation site,accounting for 59.09％.Among the strains with mutations resistant to INH molecules,a total of 8 mutation types were detected,mainly characterized by single point mutations,accounting for 96.24％.Among them,the most important mutation was katG315,accounting for 62.41％.Conclusion MTBC has a diversity of mutation types related to RIF and INH resistance,with a predominance of single point mutations,mainly in the rpoB and katG315 gene mutation sites.Deep analysis of the mutation characteristics of MTBC resistance-related genes lays a foundation for further understanding the production mechanism of drug resistance,and also provides an important reference for subsequent drug resistance prevention,control,and treatment.
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