天津市结核病控制中心156株结核分枝杆菌临床分离株耐药基因突变特征
Characteristics of drug resistance gene mutations in 156 clinical isolates of mycobacterium tuberculosis from Tianjin Center for Tuberculosis Control
王秀月 1王志锐 1穆成 1王春花1
作者信息
- 1. 天津市结核病控制中心参比实验室,天津 300011
- 折叠
摘要
目的 分析结核分枝杆菌复合群(mycobacterim tuberculosis complex,MTBC)临床分离株对利福平(rifampin,RFP)耐药突变基因rpoB和异烟肼(isoniazid,INH)耐药突变基因katG、inhA以及aphC的特征,为制定耐药结核病患者的精准治疗方案提供参考依据.方法 2021年10月—2024年1月收集天津市结核病控制中心712例结核病患者的MTBC分离株,采用荧光PCR熔解曲线法进行耐药突变的检测.结果 在712株MTBC中,共检出156株具有耐药基因突变的菌株.其中,检出88株rpoB基因的突变菌株,86株katG315基因位点突变菌株,40株inhA基因的突变菌株,以及9株aphC基因的突变菌株.在对PFP分子耐药的突变的菌株中,共检测到9种突变类型,主要以单位点突变为主,占93.18％.其中rpoB529-533是最主要的突变位点,占59.09％.在对INH分子耐药的突变的菌株中,共检测到8种突变类型,主要以单位点突变为主,占96.24％.其中,最主要的是katG315位点突变,占62.41％.结论 MTBC对RIF和INH耐药相关基因的突变类型存在多样性,以单位点突变类型为主,以rpoB和katG315基因位点突变为主,深入分析MTBC耐药相关基因突变特征,为进一步了解耐药性的产生机制奠定了基础,也为后续的耐药防控和治疗提供了参考依据.
Abstract
Objective To analyze the characteristics of resistance mutations in genes rpoB for rifampin(RFP),katG,inh A and aphC for isoniazid(INH)in clinical isolates of mycobacterium tuberculosis complex(MTBC),and provide theoretical basis for formulating precise treatment plans for drug-resistant tuberculosis patients.Methods From October 2021 to January 2024,712 MTBC isolates from tuberculosis patients in Tianjin Center for Tuberculosis Control were collected,and the drug-resistant mutations were detected by fluorescence PCR melting curve method.Results Among 712 strains of MTBC,a total of 156 strains with drug-resistant gene mutations were detected.Among them,88 strains had mutations in the rpoB gene,86 strains had mutations in the katG315 gene site,40 strains had mutations in the inhA gene,and 9 strains had mutations in the aphC gene.Among the strains with mutations resistant to PFP molecules,a total of 9 mutation types were detected,mainly characterized by single point mutations,accounting for 93.18％.Among them,rpoB529-533 was the most important mutation site,accounting for 59.09％.Among the strains with mutations resistant to INH molecules,a total of 8 mutation types were detected,mainly characterized by single point mutations,accounting for 96.24％.Among them,the most important mutation was katG315,accounting for 62.41％.Conclusion MTBC has a diversity of mutation types related to RIF and INH resistance,with a predominance of single point mutations,mainly in the rpoB and katG315 gene mutation sites.Deep analysis of the mutation characteristics of MTBC resistance-related genes lays a foundation for further understanding the production mechanism of drug resistance,and also provides an important reference for subsequent drug resistance prevention,control,and treatment.
关键词
结核分枝杆菌/耐药基因/特征Key words
Mycobacterium tuberculosis/Drug resistance gene/Characteristics引用本文复制引用
出版年
2024
NETL
NSTL
万方数据