Yulian Pills(萸连丸)Regulate the Expression of BCL-6/Blimp-1 and PD-1/PD-L1 in Spleen CD4+T Cells in the Mouse Model of Ulcerative Colitis
Objective:To investigate the modulating effects of Yulian Pills(萸连丸)on the expression of B-cell lymphoma 6(BCL-6)/B-lympho-cyte-induced maturation protein 1(Blimp-1)and programmed death receptor-1(PD-1)/programmed death ligand-1(PD-L1)in spleen CD4+T cells in the mouse model of dextran sodium sulfate(DSS)-induced ulcerative colitis(UC).Methods:Forty SPF-grade BALB/c male mice were randomized into normal control,model control,Yulian Pills(500 mg/kg),and mesalazine(300 mg/kg)groups.The mice in other groups except the normal control group were induced by DSS to establish the UC model.After successful modeling,each group was adminis-tered with corresponding drugs or saline by gavage.The body mass,colon length,colon weight,intestinal weight index,pathological morphology of colon mucosa,and histopathological damage score were determined to evaluate the therapeutic effects.The expression levels of interleukin(IL)-7,IL-9,and IL-23 in the colonic tissue were measured by enzyme-linked immunosorbent assay.The levels of CD4+BCL-6+,CD4+Blimp-1+,CD4+PD-1+,and CD4+PD-L1+cells in the mouse spleen were measured by flow cytometry.Western blotting was employed to determine the expression levels of BCL-6 and Blimp-1 in the colon tissue.Results:Compared with the normal control group,the model control group showed elevated levels of IL-7,IL-9 and IL-23 in the colon tissue(P＜0.05),increased CD4+BCL-6+,CD4+PD-1+,and CD4+PD-L1+cells(P＜0.01),reduced CD4+Blimp-1+cells(P＜0.01),up-regulated BCL-6 protein level(P＜0.05),and down-regulated Blimp-1 protein level(P＜0.01).Compared with the model control group,Yulian Pills increased the body mass and colon length(P＜0.05)and decreased the in-testinal weight index(P＜0.05).Furthermore,Yulian Pills lowered the levels of IL-7,IL-9,and IL-23 in the colon tissue(P＜0.01),reduced CD4+BCL-6+,CD4+PD-1+,and CD4+PD-L1+cells(P＜0.05),increased CD4+Blimp-1+cells(P＜0.05),down-regulated the protein level of BCL-6(P＜0.05),and up-regulated the protein level of Blimp-1(P＜0.05).Conclusion:Yulian Pills may mitigate UC by regulating the ex-pression levels of BCL-6/Blimp-1 and PD-1/PD-L1 on the surface of CD4+T cells in mice.
Yulian Pills(萸连丸)Ulcerative colitisCD4+T cellsB-cell lymphoma 6/B-lymphocyte-induced maturation protein 1Pro-grammed death receptor-1/programmed death ligand-1
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