Combination of Total Alkaloids of Huanglian(黄连)and Volatile Oil of Chuanmuxiang(川木香)Ameliorates Acne in Mice
Objective:To study the effects of the combination of total alkaloids of Huanglian(黄连)and volatile oil of Chuanmuxiang(川木香)on auricular acne in mice.Methods:The ear swelling was induced by xylene.The anti-inflammatory effects of different ratios(1∶1,1∶2,2∶1,and 4∶1)of total alkaloids of Huanglian to volatile oil of Chuanmuxiang were observed,and the optimal ratio was screened out.A mouse mod-el of acne was established by the injection of Propionibacterium acnes into the inner side of the ear.ICR mice were randomized into the follow-ing groups:blank control,model control,adapalene gel(0.06 mg/cm2),and total alkaloids of Huanglian(黄连)combined with volatile oil of Chuanmuxiang(川木香)at doses of 0.3,0.6,and 1.2 mg/cm2,with 8 mice in each group.The drugs were applied 5 days after molding,once in the morning and once in the evening,for 7 days.The auricular swelling,thymus index,and spleen index were calculated,and the his-topathological changes in the auricle were observed.The serum levels of interleukin(IL)-6,IL-1β,and interferon-γ(IFN-γ)were meas-ured,and the expression of Toll-like receptor 4(TLR4)and nuclear factor-KB p65(NF-κB p65)in the auricle was measured by immunohis-tochemistry.Results:The combination of total alkaloids of Huanglian and volatile oil of Chuanmuxiang had an anti-inflammatory effect and the optimum ratio was 4∶1.Compared with the blank control group,the model control group showed aggravated auricular swelling(P＜0.01),in-creased thymus and spleen indexes(P＜0.01),obvious pathological changes,elevated serum levels of IL-6 and IL-1 β and lowered serum IFN-γ level(P＜0.01),and increased positive expression of TLR4 and NF-κB p65 in the auricular tissue(P＜0.01).Compared with the model control group,the combination of total alkaloids of Huanglian and volatile oil of Chuanmuxiang at a ratio of 4:1 alleviated the auricular swell-ing(P＜0.05 or P＜0.01),decreased the thymus and spleen indexes(P＜0.01),mitigated the pathological changes,lowered the serum levels of IL-6 and IL-1β and elevated the serum IFN-γ level(P＜0.01),and inhibited the expression of TLR4 and NF-κB p65 in the auricular tis-sue(P＜0.01).Conclusion:The total alkaloids from Huanglian combined with the volatile oil of Chuanmuxiang demonstrate significant ther-apeutic effects on acne in mice,being capable of reducing inflammation in the mouse model of acne.
Total alkaloids of Huanglian(黄连)Volatile oil of Chuanmuxiang(川木香)Compatibility of medicinesAcneInflammation
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