首页|羌活饮片及其水提药渣的指纹图谱和抗氧化活性的谱效关系评价

羌活饮片及其水提药渣的指纹图谱和抗氧化活性的谱效关系评价

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  • NETL
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目的:建立羌活饮片及其水提药渣的UPLC指纹图谱，评价其与抗氧化活性之间的谱效关系，为羌活资源开发利用提供依据。方法:采用UPLC法测定10批羌活饮片和相应水提药渣的指纹图谱，分析样品之间相似度，结合聚类分析(HCA)和正交偏最小二乘-判别分析(OPLS-DA)，评价两者化学成分差异;采用DPPH法和ABTS法测定抗氧化指标，借助灰色关联度(Grey)和偏最小二乘回归法(PLSR)建立羌活谱-效模型。结果:对羌活饮片和药渣指纹图谱共标定18个共有峰，HCA与OPLS-DA结果一致，将饮片和药渣明显区分。所有样品具有良好的抗氧化活性，且饮片高于药渣的活性，并筛选出10个与抗氧化关联性较大的正相关峰:峰1、2、3、6、11(羌活醇)、12、13(异欧前胡素)、14、15、16峰。结论:本研究建立了基于化学成分和活性的羌活质量评价模式，并为饮片和水提药渣的质量控制和资源利用提供较为全面的参考。
Relationship between Fingerprint and Anti-Oxidative Activity of NOTOPTERYGⅡ RHIZOMA ET RADIX Decoction Pieces and Its Water-Extracted Dregs
Objective:To develop a UPLC fingerprint for NOTOPTERYGⅡ RHIZOMA ET RADIX decoction pieces and its water-extracted dregs and evaluate the relationship between the fingerprint and anti-oxidative activities,so as to provide a basis for the development and utilization of their resources.Methods:UPLC method was applied to determine the fingerprints of 10 batches of NOTOPTERYGⅡ RHIZOMA ET RADIX decoction pieces and their correlative water-extracted dregs,and then similarity was calculated.Meanwhile,hierarchical cluster analysis(HCA)and orthogonal partial least squares discriminant analysis(OPLS-DA)were employed to distinguish the chemical differences between them.The anti-oxidative indexes were examined by DPPH and ABTS,on which the spectra-effect model was established by grey relation anal-ysis(Grey)and partial least squares regression method(PLSR).Results:18 common peaks were marked out on fingerprints of NO-TOPTERYGII RHIZOMA ET RADIX decoction pieces and the dregs,which could be remarkably distinguished by HCA and OPLS-DA con-sistently.All samples performed considerable anti-oxidative abilities,while the activity of decoction pieces was higher than that of dregs.10 peaks of positive correlation with anti-oxidative activities were screened,including the peaks 1,2,3,6,11(notopterol),12,13(isoimpera-torin),14,15,and 16.Conclusions:The research has established the quality evaluation pattern based on chemical ingredients and anti-oxida-tive activities for NOTOPTERYGⅡ RHIZOMA ET RADIX,rendering a comprehensive reference on quality control and resource exploitation for Chinese decoction pieces and the water-extracted residues.

NOTOPTERYGⅡ RHIZOMA ET RADIX decoction piecesWater-extracted dregsUPLC methodSpectra-effect relationshipGrey correlation analysisPartial least squares regression method

陈仕妍、罗毓、钟文峰、吴晓英、林伟雄、汪凯东、刘燎原、陈清怡

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广东一方制药有限公司,广东省中药配方颗粒企业重点实验室,佛山 528244

羌活饮片 水提药渣 UPLC法 谱效关系 灰色关联度 偏最小二乘回归法

广东省重点领域研发计划项目

2019B110209005

2024

中药药理与临床
中国药理学会 四川省中医药科学院

中药药理与临床

北大核心
影响因子：0.996
ISSN：1001-859X
年,卷(期)：2024.40(1)
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