羌活饮片及其水提药渣的指纹图谱和抗氧化活性的谱效关系评价
Relationship between Fingerprint and Anti-Oxidative Activity of NOTOPTERYGⅡ RHIZOMA ET RADIX Decoction Pieces and Its Water-Extracted Dregs
陈仕妍 1罗毓 1钟文峰 1吴晓英 1林伟雄 1汪凯东 1刘燎原 1陈清怡1
作者信息
- 1. 广东一方制药有限公司,广东省中药配方颗粒企业重点实验室,佛山 528244
- 折叠
摘要
目的:建立羌活饮片及其水提药渣的UPLC指纹图谱,评价其与抗氧化活性之间的谱效关系,为羌活资源开发利用提供依据.方法:采用UPLC法测定10批羌活饮片和相应水提药渣的指纹图谱,分析样品之间相似度,结合聚类分析(HCA)和正交偏最小二乘-判别分析(OPLS-DA),评价两者化学成分差异;采用DPPH法和ABTS法测定抗氧化指标,借助灰色关联度(Grey)和偏最小二乘回归法(PLSR)建立羌活谱-效模型.结果:对羌活饮片和药渣指纹图谱共标定18个共有峰,HCA与OPLS-DA结果一致,将饮片和药渣明显区分.所有样品具有良好的抗氧化活性,且饮片高于药渣的活性,并筛选出10个与抗氧化关联性较大的正相关峰:峰1、2、3、6、11(羌活醇)、12、13(异欧前胡素)、14、15、16峰.结论:本研究建立了基于化学成分和活性的羌活质量评价模式,并为饮片和水提药渣的质量控制和资源利用提供较为全面的参考.
Abstract
Objective:To develop a UPLC fingerprint for NOTOPTERYGⅡ RHIZOMA ET RADIX decoction pieces and its water-extracted dregs and evaluate the relationship between the fingerprint and anti-oxidative activities,so as to provide a basis for the development and utilization of their resources.Methods:UPLC method was applied to determine the fingerprints of 10 batches of NOTOPTERYGⅡ RHIZOMA ET RADIX decoction pieces and their correlative water-extracted dregs,and then similarity was calculated.Meanwhile,hierarchical cluster analysis(HCA)and orthogonal partial least squares discriminant analysis(OPLS-DA)were employed to distinguish the chemical differences between them.The anti-oxidative indexes were examined by DPPH and ABTS,on which the spectra-effect model was established by grey relation anal-ysis(Grey)and partial least squares regression method(PLSR).Results:18 common peaks were marked out on fingerprints of NO-TOPTERYGII RHIZOMA ET RADIX decoction pieces and the dregs,which could be remarkably distinguished by HCA and OPLS-DA con-sistently.All samples performed considerable anti-oxidative abilities,while the activity of decoction pieces was higher than that of dregs.10 peaks of positive correlation with anti-oxidative activities were screened,including the peaks 1,2,3,6,11(notopterol),12,13(isoimpera-torin),14,15,and 16.Conclusions:The research has established the quality evaluation pattern based on chemical ingredients and anti-oxida-tive activities for NOTOPTERYGⅡ RHIZOMA ET RADIX,rendering a comprehensive reference on quality control and resource exploitation for Chinese decoction pieces and the water-extracted residues.
关键词
羌活饮片/水提药渣/UPLC法/谱效关系/灰色关联度/偏最小二乘回归法Key words
NOTOPTERYGⅡ RHIZOMA ET RADIX decoction pieces/Water-extracted dregs/UPLC method/Spectra-effect relationship/Grey correlation analysis/Partial least squares regression method引用本文复制引用
基金项目
广东省重点领域研发计划项目(2019B110209005)
出版年
2024
国家科技期刊平台
NSTL
万方数据