首页|肉桂挥发油化学成分及其体外抗菌、抗氧化、抗肿瘤生物活性研究

肉桂挥发油化学成分及其体外抗菌、抗氧化、抗肿瘤生物活性研究

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目的:研究肉桂挥发油化学成分组成及其体外抗氧化、抑菌和抗肿瘤等生物活性。方法:分别利用气相色谱-质谱联用(GC-MS)法和傅里叶红外光谱技术鉴定肉桂挥发油主要活性成分和官能团;紫外分光光度计测定肉桂挥发油总酚含量、DPPH自由基清除活性和β-胡萝卜素漂白抑制率;通过打孔法、微量稀释法分别测定抑菌圈直径、最小抑菌浓度(MIC)和最低杀菌浓度(MBC)，考察肉桂挥发油对常见的8种致病菌和6种耐药菌的抑菌活性;MTT法测定肉桂挥发油对不同肿瘤细胞的IC50，DCFH-DA探针法检测细胞内活性氧含量。结果:肉桂挥发油共鉴定出19种化合物，总酚含量为(29。9±1。2)mg GAE/gEO，具有较高的自由基清除能力[DPPH IC50=(4。0±0。4)μg/mL]，肉桂挥发油1。6 mg/mL时对β-胡萝卜素漂白的抑制率达68。6％;肉桂挥发油对8种常见细菌及6种耐药菌种均显示出一定的抑制作用;与空白对照组相比，肉桂挥发油对MDA-MB-231乳腺癌细胞显示出显著降低增殖、迁移、侵袭能力，显著诱导MDA-MB-231细胞凋亡(P＜0。01)。肉桂挥发油显著上调细胞内ROS水平，上调凋亡相关蛋白Caspase-3和AIF的表达(P＜0。01);引起线粒体膜电位下降，促进了细胞凋亡。结论:肉桂挥发油中成分复杂，含有多种活性成分，在抗氧化、抑菌和抗肿瘤等方面具有良好的作用。
Chemical Constituents and In Vitro Antibacterial,Antioxidant,and Antitumor Biological Activities of Essential Oil from Cinnamomum cassia Presl
Objective:To study the chemical constituents of the essential oil of Cinnamomum cassia Presl(CEO)and its in vitro biological activ-ities,including antioxidant,antibacterial,and anticancer properties.Methods:Gas chromatography-mass spectrometry(GC-MS)and Fourier-transform infrared spectroscopy(FTIR)were used to identify the main active constituents and functional groups of CEO.The total phenol content,DPPH free radical scavenging activity,and β-carotene bleaching inhibition rate of CEO were measured using UV-visible spectropho-tometry.The diameter of the inhibition zone,minimum inhibitory concentration(MIC),and minimum bactericidal concentration(MBC)were measured by drilling method and microdilution method.The antibacterial activity of CEO against eight common pathogens and six drug-resist-ant bacteria was investigated.The IC50 of CEO on different tumor cells was measured using the MTT assay,and the intracellular reactive oxy-gen species(ROS)levels were detected using the DCFH-DA probe.Results:Nineteen compounds were identified in CEO.The total phenol content was(29.9+1.2)mg GAE/g EO.CEO exhibited high free radical scavenging activity with a DPPH IC50 value of(4.0± 0.4)μg/mL.At a concentration of 1.6 mg/mL,CEO inhibited β-carotene bleaching by 68.6％.CEO showed inhibitory effects against both the eight common bacteria and the six drug-resistant bacteria.Compared with the blank control group,CEO significantly reduced the prolifera-tion,migration,and invasion abilities of MDA-MB-231 breast cancer cells,and induced apoptosis in MDA-MB-231 cells(P＜0.01).CEO significantly increased intracellular ROS levels,upregulated the expression of apoptosis-related proteins Caspase-3 and AIF(P＜0.01),de-creased mitochondrial membrane potential,and promoted cell apoptosis.Conclusion:CEO is composed of various active constituents and ex-hibits good effects in terms of antioxidant,antibacterial,and anticancer activities.

Essential oil of Cinnamomum cassia PreslAntioxidantBacteriostasisDrug-resistant bacteriaAntitumor

廖正根、温佳豪、徐希强、董伟、黄小英、钱星毅、梁新丽

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江西中医药大学现代中药制剂教育部重点实验室,南昌 330004

肉桂挥发油 抗氧化 抑菌作用 耐药菌 抗肿瘤

江西中医药大学中药制剂技术与装备创新团队江西中医药大学校级大学生创新创业训练计划项目江西中医药大学校级大学生创新创业训练计划项目江西中医药大学校级研究生创新专项资金项目

CXTD22006202210412365202210412400JZYC21S38

2024

中药药理与临床
中国药理学会 四川省中医药科学院

中药药理与临床

北大核心
影响因子：0.996
ISSN：1001-859X
年,卷(期)：2024.40(1)
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