Investigation of the Effect of Banxia Xiexin Decoction(半夏泻心汤)on Insulin Resistance in PCOS Rats from the Perspective of Intestinal Flora Diversity
Objective:To investigate the effect of Banxia Xiexin decoction(半夏泻心汤)on intestinal flora in polycystic ovary syndrome(PCOS)rats with insulin resistance(IR).Methods:The rat model of POCS with IR(PCOS IR)was established by continuous administration of letrozole by gavage.The rats were randomly divided into model group,metformin group(0.135 g/kg),Banxia Xiexin decoction group(5.31 g/kg)and Banxia Xiexin decoction+metformin group(5.31+0.135 g/kg),and normal control group.The body weight,ovarian and u-terine indexes,ovarian histopathology,serum sex hormone levels,homeostasis model assessment of insulin resistance(HOMA-IR),and intesti-nal flora detected by 16s rDNA amplicome pyrosequencing were analyzed after 28 days of continuous gavage.Results:Compared with the nor-mal control group,the model group showed significant increase in body weight,uterine index,serum testosterone(T),the ratio of luteinizing hormone(LH)/follicle-stimulating hormone(FSH),homa insulin-resistance(HOMA-IR),and blood glucose(P＜0.05),estrus disorder in rats,and a significant reduce in intestinal Chao and ACE index in rats.Furthermore,the ratio of Bacteroidetes/Firmicutes and the relative a-bundance of Prevotella were increased,while the relative abundance of Turicibacter,Lactobacillus,Akkermansia,Bifidobacterium was signifi-cantly reduced(P＜0.05 or P＜0.01).Banxia Xiexin decoction significantly reduced the body weight,uterine index,serum LH and T levels,and HOMA-IR level(P＜0.05),and increased the number,diversity and evenness of intestinal flora in PCOS model rats.In particular,the relative abundance of Firmicutes,Proteobacteria,Actinobacteria,Bacteroides,Oscillospira,Coprococcus,Roseburia,Lactobacillus,Sutterella,Bifidobacterium and Akkermansia muciniphila was increased(P＜0.05),while the relative abundance of Bacteroidetes and Cyanobacteria,Pre-votella and Anaeroplasma was reduced by Banxia Xiexin decoction(P＜0.05).Conclusion:The effect of Banxia Xiexin decoction on PCOS IR may be related to the regulation of intestinal flora,increase of the relative abundance of beneficial bacteria,decrease of the relative abundance of pathogenic bacteria,and decrease of the serum androgen and insulin levels.
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