Chinese Medical Syndrome and Nature of Isoproterenol-Induced Chronic Heart Failure Mice Based on Syndrome Determination by Formulas
Objective:The mouse model of chronic heart failure(CHF)was induced by isoproterenol(ISO).Huangqi(黄芪)Injection,a repre-sentative formula for qi(气)deficiency syndrome,Danhong(丹红)Injection,a representative formula for blood stasis syndrome,Shenmai(参麦)Injection,a representative formula for syndrome of deficiency of both qi and yin(阴),and Shenfu(参附)Injection,a representative formula for yang(阳)deficiency syndrome were administrated to explore the properties of this model from the perspective of syndrome deter-mination by formulas.Methods:The model was established by intraperitoneal injection of ISO(7.5 mg/kg).After successful modeling,mice were divided into the model group,Huangqi injection group(9.0 mL/kg),Danhong injection group(9.0 mL/kg),Shenmai injection group(9.0 mL/kg),Shenfu injection group(9.0 mL/kg),and captopril group(16.5 mg/kg),and a normal control group.After 15 d of drug in-tervention,cardiac function,NT-proBNP,TNF-α,IL-6,heart,spleen,and kidney histopathological tests were performed to explore their Chinese medical syndrome in conjunction with macroscopic phenotypes.Results:Compared with the normal control group,the LVEF,LVFS,LVPWd,LVPWs,IVSd,and IVSs values were significantly decreased in the model group(P＜0.01),and LVIDd,LVIDs,LVEDV,LVESV,NT-proB-NP,TNF-α,and IL-6 were significantly increased(P＜0.01).Compared with the model group,the values of LVIDd,LVIDs,LVEDV,LVESV,NT-proBNP,TNF-α,and IL-6 were significantly decreased in the Shenfu group and captopril group(P＜O.05 or P＜0.01),while the values of LVEF,LVFS,and LVPWd were significantly increased(P＜0.05 or P＜0.01).The efficacy of Shenfu and captopril groups in impro-ving the pathomorphology of the heart,spleen,and kidney was superior to other injections.Conclusion:The efficacy of Shenfu Injection in the mouse model with ISO-induced CHF was significant and superior to that of Huangqi,Danhong,and Shenmai injections,and it was speculated that the syndrome attribute of this model may be heart Yang deficiency syndrome.
Shenfu(参附)InjectionSyndrome determination by formulasChronic heart failureIsoproterenol
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