Effects of Bufei(补肺)Decoction on Extracellular Neutrophil Traps and Vitamin D in COPD Rats
Objective:To observe the effects of Bufei(补肺)Decoction on the components related to extracellular neutrophil traps(NETs)and the level of vitamin D in the rat model of chronic obstructive pulmonary disease(COPD)and explore the mechanism of Bufei Decoction in trea-ting COPD.Methods:Seventy SD rats were randomized into blank control,model,acetylcysteine(0.1 g/kg),vitamin D(3×10-8 g/kg),Bufei Decoction(4.5,9,and 18 g/kg)groups,with 10 rats in each group.The rats in other groups except the blank control group were mod-eled for COPD by lipopolysaccharide(LPS)combined with smoking.Hematoxylin-eosin staining was conducted to observe the pathological changes in the lung tissue.The levels of tumor necrosis factor-α(TNF-α),myeloperoxidase(MPO),25-hydroxyvitamin D3(25(OH)D3),and NETs in the serum were measured by enzyme-linked immunosorbent assay.The expression of neutrophil elastase(NE),citrullinated his-tone H3(CitH3),MPO,peptidyl arginine deiminase 4(PAD4),and vitamin D receptor(VDR)in the lung tissue was determined by West-em blotting and immunohistochemistry(IHC).Results:Compared with the blank control group,the model group showed serious symptoms and signs,disarranged structure of the lung tissue,alveolar swelling and alveolar fusion due to partial alveolar diaphragm rupture,infiltration of inflammatory cells around trachea with hyperplasia of the connective tissue.Furthermore,the modeling raised the levels of TNF-α,MPO,and NETs and lowered the level of 25(OH)D3 in the serum(P＜0.01),up-regulated the protein levels and average optical density of NE,CitH3,MPO,and PAD4 and down-regulated the protein level and average optical density of VDR in the lung tissue(P＜0.01).Compared with the model control group,acetylcysteine(0.1 g/kg),vitamin D(3×10-8 g/kg),and Bufei Decoction(9 g/kg and 18 g/kg)ameliorated the symp-toms and signs,mitigated the pathological damage in the lung tissue,lowered the levels of TNF-α,MPO and NETs and elevated the level of 25(OH)D3 in the serum(P＜0.01 or P＜0.05).At the same time,the drugs down-regulated the protein levels and average optical density of NE,CitH3,MPO,and PAD4 and up-regulated the protein level and average optical density of VDR in the lung tissue(P＜0.01 or P＜0.05).Conclusion:Bufei Decoction can reduce the inflammation and lung damage in the rat model of COPD by regulating the level of vitamin D to inhibit the release of pro-inflammatory cytokines and the expression of NET-related proteins.
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