Effect of Maodongqing(毛冬青)Tablets on Rats with Cerebral Ischemia-Reperfusion Injury and the Expressions of GFAP,NF-κBp65 and VEGFA Proteins
Objective:To observe the therapeutic effect of Maodongqing(毛冬青)Tablets on rats with cerebral ischemia-reperfusion injury and the expressions of GFAP,NF-κBp65 and VEGFA proteins,to explore the pro-angiogenic and neuroprotective effects of the prescription.Meth-ods:The MCAO/R rat model was replicated by suture method.Sixty SD rats were randomly divided into normal control group,model control group,Ginkgo biloba extract group,and Maodongqing Tablets groups(100 and 200 mg/kg).The neurological function of rats was evaluated by Longa score and balance beam score at 24 h,3 d and 7 d after modeling.The histopathological changes were detected by HE staining.Im-munofluorescence staining was used to observe the positive expressions of CD31 and VEGFA in the brain tissue around the infarct.The posi-tive co-expressions of GFAP/NeuN and NF-κBp65/NeuN were measured by immunofluorescence double staining.Western blot was performed to detect the expressions of GFAP,NF-KBp65,Phospho-NF-KBp65 and VEGF-R2 proteins in the brain tissue around the infarct.Results:Compared with the normal control group,rats in the model control group showed higher Longa scores and balance beam scores at all time points after modeling(P＜0.01),with necrotic changes in the ischemic core of the brain tissue,a significant reduction in the number of neu-rons and disorganization,and vacuolated cells.The total number of NF-KBp65-positive cells,as well as the number of nuclear translocations in the brain tissue around the infarcted foci,GFAP-positive cells,CD31 and VEGFA-positive blood vessels were increased(P＜0.05 or P＜0.01),while the number of NeuN-positive cells was decreased(P＜0.01).The expressions of NF-κBp65,Phospho-NF-κBp65,GFAP,and VEGF-R2 proteins were up-regulated(P＜0.05 or P＜0.01).Compared with the model control group,Maodongqing Tablets group(200 mg/kg)lowered the Longa score and balance beam score on the 3rd and 7th day after modeling(P＜0.01),and reduced the histopatho-logical injury in cerebral ischemia-reperfusion rats.The total number of NF-κBp65-positive cells,the number of nuclear translocations,the number of GFAP-positive cells and protein expression,and the expression of Phospho-NF-κ Bp65 protein were decreased(P＜0.05 or P＜0.01),while the number of NeuN cells and CD31-and VEGFA-positive blood vessels,and the VEGF-R2 protein expression were increased(P＜0.05 or P＜0.01).Conclusion:Maodongqing Tablets can inhibit the over-activation of astrocytes by down-regulating the GFAP protein,attenuate the inflammatory response by down-regulating the nuclear translocation and phosphorylation of NF-κBp65,and promote angiogenesis by up-regulating CD31,VEGFA and VEGF-R2 proteins.Therefore,it relieves cerebral ischemia-reperfusion injury and histopathological dam-age,and plays a neuroprotective role.
万方数据
维普
