Sorbus tianschanica Rupr.Treats Asthma by Regulating Th1/Th2 Imbalance
Objective:To investigate the anti-asthmatic effect and mechanism of Sorbus tianschanica Rupr.Methods:Sixty male SD rats of SPF grade were randomized into normal control,model,Sorbus tianschanica(1,2,and 4 g/kg),and dexamethasone(2 mg/kg)groups.The rats in other groups except the normal control group were treated with ovalbumin(OVA)by intrabitoneal injection(10％OVA)and atomization(2％OVA)for the modeling of asthma.After successfully modeling,rats in each group were treated with corresponding drugs once a day for 14 consecutive days,and the general state of the rats was observed daily.A noninvasive animal pulmonary function tester was used to measure the lung function,and the serum,bronchoalveolar lavage fluid(BALF),lung,and spleen samples were collected.The pathological changes of the lung tissue were observed by hematoxylin-eosin staining.The levels of Th1 and Th2 cells were determined by flow cytometry.Enzyme-linked immunosorbent assay was employed to determine the levels of total IgE,OVA-IgE,interferon(IFN)-γ,interleukin(IL)-4,tumor nec-rosis factor(TNF)-α,and IL-6 in the serum and BALF.Western blotting was employed to determine the protein levels of high mobility group box 1(HMGB1),Toll-like receptor 4(TLR4),myeloid differentiation factor 88(MyD88),nuclear factor-kappa B(NF-κB)p65,and inhibi-tor protein α of NF-KB(IKBα)in the lung tissue.Results:Compared with the normal control group,the model control group showed obvious asthma symptoms,increased airway hyperresponsiveness(P＜0.05),obvious lesions in the lung tissue,increased Th2 cells and decreased Th1 cells in the spleen(P＜0.05),and elevated levels of total IgE,OVA-IgE,IL-4,TNF-α,and IL-6 and lowered IFN-γ level in the serum and BALF(P＜0.05).Furthermore,the model group showed up-regulated protein levels of HMGB1,TLR4,MyD88,and NF-κB and down-regula-ted protein level of IκBα in the lung tissue(P＜0.05).Compared with the model group,S.tianschanica(1,2,and 4 g/kg)and dexametha-sone(2 mg/kg)alleviated the asthma symptoms and airway hyperreactivity(P＜0.05),mitigated the pathological changes in the lung tissue,restored the Th1/Th2 balance by decreasing Th2 cells and increasing Th1 cells in the spleen(P＜0.05),and lowered the levels of total IgE,OVA-IgE,IL-4,TNF-α,and IL-6 and elevated the level of IFN-γ in the serum and BALF(P＜0.05).Furthermore,the drugs down-regulated the protein levels of HMGB1,TLR4,MyD88,and NF-κB and up-regulated the protein level of IKBα in the lung tissue(P＜0.05).Conclu-sion:The water extract of S.tianschanica can treat asthma by regulating Th1/Th2 imbalance and inhibiting the activation of HMGB1/TLR4/NF-κB signaling pathway.
万方数据
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