Application Progress of High-Content Screening in Research on Action Mechanism and Material Basis of Traditional Chinese Medicine
Modern research on traditional Chinese medicine has been characterized by the complex and incomplete screening of material bases of ef-ficacy and the abundance and insufficient understanding of targets of the action mechanism,which has led to uncertainties and inadequacies in the research results and prevented them from being internationally recognized.High-content screening(HCS),also known as high-content a-nalysis(HCA),is a high-throughput,multi-target,multi-channel,and fully automated analysis technique that can quantify the specific re-sponse image of a large number of cells to a drug.It can be used in the modern research on traditional Chinese medicine to match the multi-target actions of traditional Chinese medicine and its compounds and study their action mechanisms and material bases,so as to improve the efficacy of traditional Chinese medicine,expand the scope of drug use,ensure the safety of drug use,and make the results internationally rec-ognized.This paper briefly introduces the principle of HCS and its application progress in the research on the action mechanism and material basis of traditional Chinese medicine,and the paper considers its advantages and limitations and its contribution to modern research on tradi-tional Chinese medicine through examples.
High-content analysisHigh-content screeningTraditional Chinese medicineMaterial basisAction mechanismSignal acquisi-tionSafety evaluation
万方数据
维普
