Comparison of the in vitro Effects of Qingfei Paidu Decoction(清肺排毒汤),Huashi Baidu Decoction(化湿败毒方)and Xuanfei Baidu Decoction(宣肺败毒方)against Complement Activation
Comparison of the in vitro Effects of Qingfei Paidu Decoction(清肺排毒汤),Huashi Baidu Decoction(化湿败毒方)and Xuanfei Baidu Decoction(宣肺败毒方)against Complement Activation
Objective:To compare the effects of three decoctions for COVID-19,Qingfei Paidu Decoction(清肺排毒汤,QF),Huashi Baidu Decoc-tion(化湿败毒方,HS),Xuanfei Baidu Decoction(宣肺败毒方,XF)against the complement activation(the important pathogenesis of COVID-19),by using the parallel experimental models.Methods:Based on the principle of ELISA,the IgM,zymosan and mannan were se-lected as coating substances to activate the classical,alternative and lectin complement pathway,respectively.Complement activation was eval-uated by measuring the level of terminal complement complex C5b-9.Meanwhile,coated SARS-CoV-2 spike protein(S protein)and SARS-CoV-2 nucleocapsid(N protein)was employed to investigate the effects of three decoctions against complement activation mediated by these two proteins.Results:Three decoctions for COVID-19 could concentration-dependently inhibit the production of C5b-9 in three complement activation pathways,and they all inhibited the C5b-9 production triggered by S and N proteins.Among them,XF exhibited the strongest abili-ty.Conclusion:Three decoctions for COVID-19 can suppress the complement activation mediated by all three pathways,as well as S and N proteins in vitro,suggesting that the ability of three decoctions against complement activation may be one of the pharmacodynamic bases for their clinical use in the treatment of COVID-19.
维普
万方数据