Effect of Traditional Chinese Medicine Master Ban Xiuwen's Huancao Decoction (欢草汤) on Sex Hormone and Expression of Sex Hormone Receptor in Rats with Hyperplasia of Mammary Glands
Objective:To explore the therapeutic effect and therapeutic mechanism of Huancao Decoction (欢草汤) on rats with hyperplasia of mammary glands.Methods:A total of 10 of the 80 rats were randomly selected as the normal control group,while the rest rats were made into the model of hyperplasia of mammary glands.The successfully modeled rats were randomly divided into model control group,Huancao Decoc-tion (24.2,12.1,and 6.1 g/kg) groups,Huancao Decoction (12.1 g/kg) preventive treatment group,and Tamoxifen (4 mg/kg) group.Af-ter four weeks of continuous treatment,the appearance of the breasts of rats in each group was observed,and the height and diameter of the nipples were measured.Pathological morphology of breast tissue was observed by hematoxylin-eosin (HE) staining.The levels of serum gon-adotropin-releasing hormone (GnRH),follicle-stimulating hormone (FSH),luteinizing hormone (LH),prolactin (PRL),estradiol (E2),pro-gesterone (P),and testosterone (T) were measured by enzyme-linked immunosorbent assay (ELISA).The protein expressions of estrogen receptor alpha (ERα),estrogen receptor beta (ERβ),progesterone receptor (PR),and prolactin receptor (PRLR) in breast tissue were measured by Western Blot (WB).The mRNA expressions of estrogen receptor alpha (Esr1),estrogen receptor beta (Esr2),progesterone (Pgr),progesterone receptor A (Pgra),progesterone receptor B (Pgrb),and prolactin receptor (Prlr) in breast tissue were detected by RT-qPCR.Results:Compared with the normal control group,the levels of serum E2 and PRL in the model control group were significantly in-creased,while the level of P was significantly reduced (P<0.01).In breast tissue,the protein expressions of ERα,PR,and PRLR were up-regulated significantly,while ERβ was downregulated significantly (P<0.01).The mRNA expressions of Esr1,Pgr,Pgrb,and Prlr in breast tissue were upregulated significantly,while those of Esr2 and Pgra were significantly downregulated (P<0.01 or P<0.05).After treatment with Huancao Decoction,the above indexes were significantly reversed (P<0.01 or P<0.05).Conclusions:Huancao Decoction may treat hy-perplasia of mammary glands by reducing serum E2 and PRL,increasing P level,inhibiting ERα,PR,and PRLR,promoting ERβ protein ex-pression in breast tissue,inhibiting Esr1,Pgr,Pgrb,and Prlr,and improving Esr2 and Pgra mRNA expressions in breast tissue.
Huancao Decoction (欢草汤)Hyperplasia of mammary glandsSex hormoneSex hormone receptor
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