Effect of MUME FRUCTUS on Rat Model of Pixuxiexie (脾虚泄泻) Syndrome
Objective:To investigate the anti-diarrheal effect and mechanism of MUME FRUCTUS on the Pixuxiexie syndrome (PXXX) model rats.Methods:The PXXX rat model was established by gavage with RHEI RADIX ET RHIZOMA decoction.MUME FRUCTUS decoction or mont-morillonite powder was given for 5 days.The general conditions of the rats were observed,and Bristol stool form scale,fecal consistency,fecal water content,small intestinal propulsion rate,and gastric residue rate were measured.Serum motilin (MLT),gastrin (GAS),and colonic Na+-K+-ATPase content were measured.Colonic pathological sections were observed,and immunofluorescence was used to detect the expres-sion of colonic aquaporin Protein-3 (AQP3) and AQP4.Results:Compared with the normal control group,the model control group showed significant loose stools,with significantly increased Bristol stool form scale,fecal consistency,and fecal water content,while body weight signif-icantly decreased,small intestinal propulsion rate significantly increased,and gastric residue rate significantly decreased (P<0.01).Serum MLT content increased significantly (P<0.05),serum GAS content decreased significantly (P<0.01),colonic Na+-K+-ATPase content de-creased significantly (P<0.01),obvious inflammation infiltration changes,mucosal defects,and glandular structure separation were observed under light microscope,and the relative expression levels of colonic AQP3 and AQP4 proteins decreased significantly (P<0.01).Compared with the model control group,each dose group of MUME FRUCTUS showed improvement diarrhea in PXXX model,with significantly decreased Bristol stool form scale,fecal consistency,and fecal water content (P<0.01),significantly decreased small intestinal propulsion rate,signifi-cantly increased gastric residue rate (P<0.01),significantly decreased serum MLT and GAS content (P<0.05 or P<0.01),significantly in-creased colonic Na+-K+-ATPase content,significantly increased colonic AQP4 protein expression (P<0.01),proliferation of colonic tissue and improvement of mucosal damage were observed under light microscope.The body weight of rats in the MUME FRUCTUS decoction 10 g/kg group increased significantly (P<0.05),and the expression of colonic AQP3 in the MUME FRUCTUS decoction 2.5 g/kg and 5 g/kg groups increased significantly (P<0.05 or P<0.01).Conclusion:MUME FRUCTUS has a significant anti-diarrheal effect on PXXX rats.Its specific mechanism is related to the reduction of MLT,GAS content,inhibition of gastrointestinal hyperactivity,regulation of Na+-K+-ATPase activity,enhancement of AQP3,AQP4 expression,and reduction of fecal water content.It also improves mucosal damage,inflamma-tion infiltration,and other manifestations.
MUME FRUCTUSRelieving diarrhea with astringentsPixuxiexie (脾虚泄泻)AQP
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