Formulation Optimization and Extraction Technology of Wuwei Shenqin Decoction (五味参芩汤) Based on Pulmonary Fibrosis Model
Objective:To explore the optimal extraction process of Wuwei Shenqin Decoction (五味参芩汤,WSD),to screen the optimal propor-tion of WSD based on a rat model of pulmonary fibrosis.Methods:High-performance liquid chromatography (HPLC) and orthogonal assay were used to explore the optimal extraction process of WSD,and bleomycin was used to establish a rat model of pulmonary fibrosis to screen the optimal proportion of WSD.The pathological changes of lung tissues were observed by HE staining.The DSI/BUXCO airway resistance and lung compliance monitoring system was used to monitor and record the tidal volume,airway resistance,and dynamic lung compliance of rats in real time.Kits were used to detect the levels of procollagen type Ⅲ aminoterminal propeptide (PIIINP),matrix metalloproteinase 7 (MMP-7),surfactant protein A (SP-A),surfactant protein D (SP-D),Krebs von den Lungen-6 (KL-6),heme oxygenase-1 (HO-1) in ser-um,and hydroxyproline (HYP) in lung tissue of model rats with pulmonary fibrosis.The comprehensive score of each group was calculated to select the optimal proportion from the 16 combinations.With the content of ginsenoside Rg1,baicalin,schisandrin A,ferulic acid,and platyc-odon D in WSD and the extraction rate as comprehensive indexes,the L9 (34) orthogonal experiment table was designed with ethanol concen-tration,liquid-to-material ratio,decocting time,and decocting times as factors,and the comprehensive score of each group was calculated to op-timize the extraction process of WSD.Results:Compared with the results in the blank control group,the tidal volume and dynamic lung com-pliance of the model control group were significantly decreased (P<0.01),while airway resistance and the content of PIIINP,MMP-7,SP-A,SP-D,KL-6,HO-1,and HYP were significantly increased (P<0.01).Compared with the results in the model control group,the tidal volume and dynamic lung compliance were significantly increased (P<0.05,P<0.01),while the airway resistance and content of PIIINP,MMP-7,SP-A,SP-D,KL-6,HO-1,and HYP were significantly decreased (P<0.05,P<0.01).The optimal proportion of WSD was Ginseng Radix et Rhizoma 9 g,Scutellariae Radix 15 g,Schisandrae Chinensis Fructus 6 g,Chuanxiong Rhizoma 12 g,and Platycodonis Radix 9 g.The optimal extraction process was as follows:10 times the amount of 90％ ethanol was used for decocting for 1.5 hours with once extraction.Conclusion:The rat model of pulmonary fibrosis was successfully established using bleomycin,and WSD can be used in the treatment of pulmonary fibro-sis.In addition,the optimal proportion of WSD was selected,and the liquid-phase method for determining the main components in this formula was established.The optimal extraction process shows good reproducibility,stability,precision,sample addition,and recovery,and the method is stable and reliable.
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