Effect of Modified Shaoyao(芍药)-Gancao(甘草)Decoction on Migration,Invasion,and Angiogenesis of Endometrial Stromal Cells in Ectopic Adenomyosis
Objective:To investigate the effect of modified Shaoyao(芍药)-Gancao(甘草)Decoction on migration,invasion,and angiogenesis of endometrial stromal cells(EScs)in ectopic adenomyosis(AM)through in vitro experiments.Methods:Primary EScs were collected,cul-tured,and isolated,and cell immunohistochemistry was used to identify EScs.Transwell migration and invasion tests were conducted to detect the effect of modified Shaoyao-Gancao Decoction on the migration and invasion ability of EScs.The mRNA expressions of angiogenesis-related factors in EScs were detected by real-time quantitative polymerase chain reaction(RT-qPCR).Microvascular density(MVD)in the cells was labeled by immunofluorescence.Results:Six human AM primary cells that were collected,cultured,and isolated were all EScs.Compared with those in the control group,the cell migration and invasion ability in the group containing 10％of 1.69 g/kg modified Shaoyao-Gancao Decoc-tion and dienogest group were significantly decreased(P＜0.01),the mRNA expressions of Vegf,Hif-1α,Pdgf,Ang,Fgf1,and Fgf2 in the group containing 10％of modified Shaoyao-Gancao Decoction were significantly down-regulated(P＜0.05 or P＜0.01).Immunofluorescence of MVD was significantly decreased(P＜0.05),and mRNA expressions of Vegf,Pdgf,Ang,and Fgf1 in the dienogest group were significantly decreased(P＜0.05 or P＜0.01).Compared with those in the normal control group,the cell migration and invasion ability in the group contai-ning 10％of modified Shaoyao-Gancao Decoction and dienogest group were significantly decreased(P＜0.05 or P＜0.01).The mRNA expres-sions of Fgf1 and Fgf2 in the group containing 10％of modified Shaoyao-Gancao Decoction were significantly down-regulated(P＜0.05),and the mRNA expression of Ang in the dienogest group was significantly down-regulated(P＜0.05).Conclusion:EScs were collected,and modi-fied Shaoyao-Gancao Decoction could inhibit the migration and invasion of EScs,as well as the expression of angiogenesis-related factors.
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