Effects of CORNI FRUCTUS-DIOSCOREAE RHIZOMA Aqueous Extract on Immune Function and Intestinal Flora in Cyclophosphamide-Induced Immunosuppressed Mice
Objective:To investigate the effects of CORNI FRUCTUS-DIOSCOREAE RHIZOMA aqueous extract on the immune function and intesti-nal flora in cyclophosphamide-induced immunosuppressed mice.Methods:The mice were randomly divided into a normal control group,a model group,a 0.08 g/kg levamisole tablets group,and 0.43,0.86,and 1.73 g/kg CORNI FRUCTUS-DIOSCOREAE RHIZOMA aqueous ex-tract groups,with 10-11 mice in each group.Except for the normal control group,mice in the other groups were intraperitoneally injected with 40 mg/kg cyclophosphamide on the first three days.From the 4th day,the corresponding drugs were given by gavage for 14 consecutive days.After 24 hours of the last administration,the organ indexes,the percentage of NK cells in the spleen,the proportions of T and B lymphocyte subsets in peripheral blood of mice in each group were determined,and the changes in intestinal flora in mice of each group were analyzed.Results:Compared with those of the normal control group,the body weight,thymus index,spleen index,CD8+T,CD19,and CD 19/CD 13 were significantly decreased in the model control group,while the percentage of NK cells was significantly increased(P＜0.05 or P＜0.01).Com-pared with those of the model control group,NK lymphocytes in the levamisole tablets group were significantly decreased(P＜0.01),while the CD19/CD13 ratio was significantly increased after the intervention by 1.73 g/kg CORNI FRUCTUS-DIOSCOREAE RHIZOMA aqueous ex-tract(P＜0.05).Compared with the normal control group,the model control group showed a significantly decreased Shannon index(P＜0.05),which reflected the alpha diversity of intestinal flora,significantly decreased Verrucomicrobia(P＜0.01),and significantly increased Firmicutes(P＜0.05).In comparison with the model control group,the 0.43 g/kg CORNI FRUCTUS-DIOSCOREAE RHIZOMA aqueous ex-tract group saw significantly increased Verrucomicrobia(P＜0.01),the 0.86 g/kg CORNI FRUCTUS-DIOSCOREAE RHIZOMA aqueous ex-tract group witnessed significantly decreased Firmicutes(P＜0.05),the 0.08 g/kg levamisole tablets group showed significantly increased Bacteroidetes(P＜0.05).In terms of genus classification,compared with those of the model control group,Odoribacter and Alistipes in the 0.08 g/kg levamisole tablets group were significantly increased(P＜0.05,P＜0.01);Odoribacter was significantly increased(P＜0.05),while Lachnoclostridium was significantly decreased(P＜0.01)in the 0.86 g/kg CORNI FRUCTUS-DIOSCOREAE RHIZOMA aqueous ex-tract group,Lachnoclostridium was significantly decreased in the 1.73 g/kg CORNI FRUCTUS-DIOSCOREAE RHIZOMA aqueous extract group(P＜0.01).Conclusion:CORNI FRUCTUS-DIOSCOREAE RHIZOMA aqueous extract can improve the immune function and intestinal flora dysfunction induced by cyclophosphamide in mice.
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