Pathogenesis of Nonalcoholic Steatohepatitis with Sarcopenia
Objective:The incidence of nonalcoholic steatohepatitis(NASH)with sarcopenia is rising,while the mechanism underlying this condi-tion has not been systematically elucidated.This article aims to reveal the internal relationship between NASH and sarcopenia by animal mod-els and provide research tools and a theoretical basis for clinical practice and new drug development.Methods:A three-factor two-level or-thogonal design was adopted to evaluate the effects of castration and dexamethasone on the formation of sarcopenia in mice based on blood,liv-er,and muscle biochemical indexes and histopathological observation.Results:The mice subjected to testicular resection showcased decreased liver and muscle indexes,prolonged freezing time,elevated levels of total bile acid(TBA),triglycerides(TG),low-density lipopolysaccharide cholesterol(LDL-C),and glucose(Glu)in the serum,TG and total cholesterol(TC)in the liver,and malondialdehyde(MDA),TG,and lactate dehydrogenase(LDH)in the muscle,and lowered levels of alanine transaminase(ALT)and aspartate transaminase(AST)in the ser-um.Ovariectomy of mice reduced the muscle index,prolonged the freezing time,and elevated the levels of hydroxyproline in the liver and pyruvate in the muscle.Long-term injection of high-dose dexamethasone induced significant decreases in muscle index and muscle strength,elevated the levels of ALT,TG,TC,HDL-C,LDL-C,and creatine(Crea)in the serum,TG in the muscle,and TG and TC in the liver,and lowered the glycogen level in the liver.Intraperitoneal injection of dexamethasone induced changes similar to NASH with sarcopenia,while it lowered the lactic acid level in the muscle in ovariectomized mice.Conclusion:The decreases in sex hormones and dexamethasone demon-strate synergistic enhancement effect in the formation of NASH with sarcopenia in male animals.NASH with sarcopenia is a systemic reaction of NASH,and its occurrence and development are closely related to insulin resistance,sex hormone levels,and protein and lipid metabolism levels.
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