Regulatory Effects and Mechanism of Yinchen Qingfei Granules on Mycoplasma Pneumoniae Pneumonia in Mice Based on the TLR2/NF-κB Signaling Pathway
Objective:To investigate the mechanism and effective targets of Yinchen Qingfei Granules in treating Mycoplasma Pneumoniae Pneumonia(MPP).Methods:Thirty-six SPF-grade BALB/c mice were used.After 3 days of adaptive feeding with standard diet,6 mice were randomly selected as the blank group,and the remaining 30 mice were subjected to nasal instillation of Mycoplasma Pneumoniae(MP)bacterial solution combined with high-fat diet and environmental exposure to establish a model of damp-heat obstructing lung syndrome.After the model was successfully established,the mice were randomly divided into model group,azithromycin group(administered azithromycin 90.1 mg·kg-1·d-1),and low(8.109 g·kg-1·d-1),medium(5.406 g·kg-1·d-1),and high-dose(2.703 g·kg-1·d-1)Yinchen Qingfei Granules groups.The model and blank groups were given an equal volume of 0.9％saline.After 3 days of treatment,tissues were collected 4 hours after the last dose for analysis.HE staining was used to observe the pathological changes in the lung tissue of the mice;RT-qPCR was used to detect the mRNA expression of TLR2,MyD88,and NF-κBp65 in lung tissue;Western blot was used to detect the protein expression levels of TLR2,MyD88,and NF-κBp65 in lung tissue.Results:HE staining showed that the alveolar structure was clear in the blank group,while the model group exhibited lung consolidation,extensive inflammatory cell infiltration,and significant hemorrhage in the vessels.Lung tissue lesions,cellular infiltration,and inflammation were improved in all treatment groups.Compared to the blank group,the mRNA expression of TLR2,MyD88,and NF-κBp65 significantly increased in the model group(P＜0.01).Compared to the model group,mRNA expression levels of TLR2 and NF-κBp65 were significantly decreased in all treatment groups except the low-dose group(P＜0.05),with the high-dose Yinchen Qingfei Granules group having lower TLR2 mRNA expression than the azithromycin group(P＜0.05).Compared to the blank group,the protein expression levels of TLR2,MyD88,and NF-κBp65 were significantly increased in the model group(P＜0.05).Except for the medium-dose Yinchen Qingfei Granules group where MyD88 and NF-κBp65 protein levels were not significantly different,other treatment groups had significantly lower pathway protein expression than the model group(P＜0.05).Conclusion:Yinchen Qingfei Granules can significantly improve inflammatory symptoms in MP-infected mouse lung tissue,and its mechanism may be related to the inhibition of the TLR2/NF-κB signaling pathway.
TLR2/NF-κBMycoplasma penumoniae PneumoniaDampness-dispelling and collaterals-unblockingYinchen Qingfei Granules
万方数据
维普
