Exploration of the Anti-Inflammatory Mechanism of Jiawei Guomin Decoction on Antibiotic-Exacerbated Allergic Asthma in Mice Based on the TLR4/MyD88/NF-κB Pathway
Objective:To investigate the intervention effect and potential mechanism of Jiawei Guomin Decoction on antibiotic-exacerbated allergic asthma in mice.Methods:Forty male BALB/c mice were randomly divided into four groups:a blank group,a model group(antibiotic-exacerbated allergic asthma group),a traditional Chinese medicine group(Jiawei Guomin Decoction group,6.3 g·kg-1),and a Western medicine group(Pidotimod 0.0378 g·kg-1+theophylline 0.2 g·kg-1),with 10 mice per group.Allergic asthma mouse model was established using ovalbumin(OVA)and aluminum hydroxide gel adjuvant combined with OVA nebulization,and antibiotic-exacerbated allergic asthma model was established by nasal instillation of cefoperazone and sulbactam sodium.Drugs were administered by gavage 30 minutes before nebulization for 7 days,and the mice were then assessed for lung tissue pathology.ELISA was used to measure serum levels of IgE,IL-4,IL-5,and IL-13;Western blot was used to detect protein expression levels of TLR4,MyD88,and p-NF-κBp65 in lung tissue.Results:Compared to the normal group,the antibiotic-exacerbated allergic asthma group exhibited severe abnormalities in bronchial structure,shedding of some bronchial epithelial cells,significant protein mucus exudation,extensive inflammatory cell infiltration around the bronchi,thickened alveolar walls,and alveolar atrophy.Serum levels of IgE,IL-4,IL-5,and IL-13 were elevated(P＜0.01);TLR4,MyD88,and p-NF-κBp65 protein expressions were increased(P＜0.01).Compared to the antibiotic-exacerbated allergic asthma group,the Jiawei Guomin Decoction group showed normal bronchial structure,significantly improved alveolar wall thickening,and reduced inflammatory cell infiltration.Serum levels of IgE,IL-4,IL-5,and IL-13 were significantly decreased(P＜0.01);TLR4,MyD88,and p-NF-κBp65 protein expressions were markedly reduced(P＜0.01).Conclusion:Jiawei Guomin Decoction can improve airway inflammation in antibiotic-exacerbated allergic asthma,and its mechanism may be related to the regulation of the TLR4/MyD88/NF-κB signaling pathway.
万方数据
维普
