Objective:To establish HPLC fingerprint of Cyclocarya paliurus and provide a theoretical basis for its quality evaluation.Methods:By using RP-HPLC method,column chromatography was Merck C18 column (250 mm× 4.6 mm,5 μm),acetonitrile-0.1％ phosphoric acid solution gradient elution by flow volume of 1.0 mL · min-1,column temperature at 35 ℃ and the detection wavelength at 245 nm.With the State Pharmacopoeia Commission Similarity Evaluation Software (2004A) processing and analysis,establish Cyclocarya paliurus HPLC fingerprint.Results:There were 32 common peaks.The fingerprint chromatography of 10 batches of cyclocarya paliurus were better,in line with the fingerprint.Conclusions:This method is simple and reliable and it provides a scientific basis for the quality control of Cyclocarya paliurus.
NETL
NSTL
万方数据
维普
