Protective Effect of Hongjing Qingfei Prescription(红景清肺方)on PM2.5-induced Lung Injury in Mice Based on Energy Metabolism
Objective This paper investigated the protective effect and mechanism of Hongjing Qingfei Prescription(红景清肺方)on PM2 5-induced lung injury in mice.Methods Totally 40 ICR male mice were randomly assigned into a blank control group,a PM2.5 model group,a traditional Chinese medicine equivalent group and a traditional Chinese medicine double group.Af-ter 7 days of pre-administration of traditional Chinese medicine,except the blank control group,the other group was given PM2.5 suspension(0.02 mg·μL-1)continuously for 2 days to prepare a mouse lung injury model through nasal drip.24 hours after the last exposure,the lung tissue samples were taken out and Hematoxylin eosin(HE)staining was used to observe the mor-phological changes of lung tissue;Enzyme linked immunosorbent assay(ELISA)was used to detect the contents of adenosine triphosphate(ATP),Na+-K+-ATPase and Ca2+-Mg2+-ATPase.Western blot was used to detect AMP activated protein kinase(AMPK)and peroxisome proliferator activated receptor 1 α(PGC-1α)and the expression leve of mitochondrial transcrip-tion factor A(TFAM)protein.Immunohistochemistry(IHC)was used for detecting protein expressions of PGC-1α,TFAM and nuclear respiratory factor-1(Nrf1).Results Compared with those of the blank control group,the pathological injury of lung tis-sue structure in the PM2 5 model group was obvious,the contents of ATP,Na+-K+-ATPase and Ca2+-Mg2+-ATPase in lung tissues were significantly reduced(P＜0.01),and the expression levels of AMPK,PGC-1α and TFAM proteins and the positive expression rates of PGC-1α,Nrf1 and TFAM proteins were significantly reduced(P＜0.01).Compared with those of the PM2 5 model group,the lung tissue injury in traditional Chinese medicine equivalent group and traditional Chinese medicine double group was significantly improved.The content of ATP in lung tissue in traditional Chinese medicine double group increased(P＜0.05),and the contents of Na+-K+-ATPase and Ca2+-Mg2+-ATPase increased significantly(P＜0.01).Although the contents of ATP,Na+-K+-ATPase and Ca2+-Mg2+-ATPase also increased in traditional Chinese medicine equivalent group,the difference was not statistically significant(P＞0.05).The expressions of AMPK,PGC-1α and TFAM proteins and the positive expression rates of PGC-1α,Nrf1 and TFAM proteins were significantly increased in both traditional Chinese medi-cine equivalent group and traditional Chinese medicine double group(P＜0.01).Conclusion Hongjing Qingfei Prescription can improve the activities of Na+-K+-ATPase and Ca2+-Mg2+-ATPase in lung tissue,up-regulate the level of AMPK/PGC-1α signaling pathway protein,reduce mitochondrial injury and dysfunction,promote mitochondrial biosynthesis,effectively improve lung tissue energy metabolism and reduce PM2 5 injury to lung tissue.
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